2015

1. Wojnarowska R., Polit J., Broda D., Gonchar M., Sheregii E.M. Surface enhanced Raman scattering as a probe of cholesterol oxidase enzyme // Appl. Physics Letters. – 2015. – V. 106, 103701. doi: 10.1063/1.4914499 (IF=3.515)
2. Stasyk O., Boretsky Y., Gonchar M., Sibirny A. Recombinant arginine-degrading enzymes in metabolic anticancer therapy and biosensorics // Cell Biol. Intern. - 2015. - V. 39, N 3. – P. 246-252 (IF=1.640)
3. Stasyuk N.Ya., Gayda G.Z., Serkiz R.Ja., Gonchar M.V. Cell Imaging with Fluorescent Bi-Metallic Nanoparticles // Journal of Advances in Chemistry. - 2015. - V. 11, No. 4. – P. 3498–3510 (IF=1.415)
4. Sibirnyj W., Grabek-Lejko D., Gonchar M. The use of enzymes for ethanol, methanol and formaldehyde determination in food products // Journal of Microbiology, Biotechnology and Food Sciences. – 2015. – V. 4, No 5. – P. 393-397.

2016

1. Karkovska M., Smutok O., Gonchar M. Laboratory prototype of bioreactor for oxidation of toxic D-lactate using yeast cells overproducing D-lactate-cytochrome c oxidoreductase // BioMed Research Intern. – 2016. – P. 1-5. (IF=2.134)
2. Lesanavičius M., Smutok O., Valiauga B., Marozienė A., Gonchar M., Krikštopaitis K., Čėnas N. Kinetic properties of flavocytochrome b₂ from Hansenula polymorpha // Chemija. – 2016. - V. 2, N 2. - P. 23-127. (IF=0.472)
3. Stasyuk N., Gayda G., Gonchar M. Novel arginine deiminase-based method to assay L-Arginine in beverages // Food Chemistry. – 2016. – V. 201. – P. 320-326 (IF=4.052)
4. Stasyuk N., Gayda G., Yepremyan H., Stepien A., Gonchar M. Fluorometric enzymatic assay of L-arginine // Spectrochim. Acta Part A: Molecular and Biomolecular Spectroscopy. - 2017. - V. 170. – P. 184–190. (available online 11 July 2016) (IF=2.653)
5. Stasyuk N., Gayda G., Klepach H., Gonchar M. Nanoparticles of noble metals as effective platforms for the fabrication of amperometric biosensor on hydrogen peroxide // Sensor Letters. – 2016. – V. 14, N 11-12. – P. 1–9 (IF=0.320)
6. Stasyuk N., Synenka M., Gayda G., Smutok O., Gonchar M. Arginase-based amperometric biosensor for manganese ions analysis. // “Eureka. Life science”. – 2016. – N 1. – P. 22-28
7. Stasyuk N., Gayda G., Serkiz R., Gonchar M.. The “green” synthesis of gold nanoparticles by the yeast Hansenula рolymorpha // Вісник Львівського університету. – Вип.73. – С. 96-102
8. L.V. Kobrina, V.V. Boiko, T.V. Dmitrieva, V.V. Bortnitsky, T.M. Prokopiv, S.V. Riabov, M.V. Gonchar. Influence of physical factors and cultures of microorganisms on segmented polyurethane’s degradation // Polimer. Journal. - 2016. – V.38, N3. – P. 236-243
9. O. Demkiv, S. Banah, H. Klepach, G. Gayda, O. Smutok, M. Gonchar. Fungal oxidases: screening of strains and perspectives of bioanalytical application // Visnyk of the Lviv University. Series Biology. 2016. Issue 73. P. 55–64
10. Demkiv O., Banah S., Prytula S., Gayda G., Gonchar M. Screening of fungal strains – producers of laccase // ScienceRise:Biological Science. - 2016. - № 3 (3). - P. 66-69
11. Karkovska M., Smutok O., Gonchar M. Usage of flavocytochromeb2 immobilized onto magnetic microparticles in multiple enzymatic analysis of L-lactate // Visnyk of the Lviv University. Series Biology. 2016. Issue 72. P. 25-32

2017

1. Demkiv O., Smutok O., Gonchar M., Nisnevitch M. Reagentless amperometric formaldehyde-selective chemosensor based on using platinized gold electrode // Materials. – 2017. - V.10, N.5 - E503 (1-11) doi:10.3390/ma10050503 (IF=2.654)
2. Gayda G., Gonchar M., Sibirny A. Yeast-based systems for environmental control // In the Book "Biotechnology of Yeasts and Filamentous Fungi" (Ed. A.A. Sibirny). – Springer, 2017. – P. 373-386
3. Gayda G.Z., Stasyuk N.Ye, Serkiz R.Ja, Gonchar M.V. Fluorescent nanoparticles of noble metals for cell imaging // В книзі: “Багатофункціональні наноматеріали для біології і медицини: молекулярний дизайн, синтез і застосування” (ред. Р.С. Стойка) – 2017. – C. 156-165
4. Gonchar M., Smutok O., Karkovska M., Stasyuk N. Yeast-based biosensors for clinical diagnostics and food control // In the Book "Biotechnology of Yeasts and Filamentous Fungi" (Ed. A.A. Sibirny). - Springer, 2017. – P. 392-400
5. Kavetskyy T., Smutok O., Gonchar M., Demkiv O., Klepach H., Kukhazh Y., Šauša O., Petkova T., Boev V., Ilcheva V., Petkov P., Stepanov A.L. Laccase-containing ureasil-polymer composite as the sensing layer of an amperometric biosensor // Journal of Applied Polymer Science. – 2017. – V.134 (36). – P. 45278-45285 (IF=1.866)
6. Kavetskyy T., Šauša O., Čechová K., Švajdlenková H., Mat’ko I., Petkova T., Boev V., Ilcheva V., Smutok O., Kukhazh Y. and Gonchar M. Network Properties of Ureasil-Based Polymer Matrixes for Construction of Amperometric Biosensors as Probed by PALS and Swelling Experiments // Acta Physica Polonica A. - 2017. - V. 132, N 5. - P. 1515-1519 (IF=0.469)
7. Kovalyshyn Y., Konovska M., Milanese C., Saldan I., Serkiz R., Pereviznyk O., Reshetnyak O., Kuntyi O. Electrochemical properties of the composites synthesized from polyaniline and modified MWCNT // Chem. Chem. Technol., – 2017, – V. 11, N. 3, – P. 261-269 (in Scopus)
8. Smutok O., Karkovska M., Serkiz Ya., Vus B., Čenas N., Gonchar M. A Novel Mediatorless Biosensor Based On Flavocytochrome b₂ Immobilized Onto Gold Nanoclusters For Non-invasive L-lactate Analysis Of Human Liquids // Sensor & Actuators B. – 2017. - V. 250. - P. 469-475, DOI: 10.1016/j.snb.2017.04.192 (IF=5.401)
9. Stasyuk N., Gayda G., Serkiz R., Gonchar M. The “green” synthesis of gold nanoparticles by the yeast Hansenula рolymorpha // Вісник Львівського університету. Серія біологічна. - 2016. – Вип. 73. - С. 96-102
10. Stasyuk N., Gayda G., Yepremyan H., Gonchar M. Fluorometric enzymatic assay of L-arginine // Spectrochimica Acta. Part A. – 2017. – V. 170. – P. 184-190 (IF=2.653)
11. Stasyuk, N., Gayda, G., Klepach, H., Semashko, T., Gonchar, M., 2017. Nanoparticles of noble metals for enzymatic sensors: an amperometric glucose biosensor for wine analysis // Sensor Letters. – 2017 – V.15, N8. – P. 647-654 (IF=0.558)
12. Stepien A.E.,  Zebrowski J., Piszczyk Ł., Boyko V.V.,   Riabov S.V., Dmitrieva T.,  Bortnitskiy V.I.,  Gonchar M., Wojnarowska-Nowak R., Ryszkowska J. Assessment of the impact of bacteria Pseudomonas denitrificans, Pseudomonas fluorescens, Bacillus subtilis and yeast Yarrowia lipolytica on commercial poly(ether urethanes) // Polymer Testing. – 2017. – V.63. – P.484-493. (IF=2.464)
13. Toporovska L.R., Hryzak A.M., Turko B.I., Rudyk V.P., Tsybulskyi V.S., Serkiz R.Y.. Photocatalytic properties of zinc oxide nanorods grown by different methods // Optical and Quantum Electronics, – 2017, – Vol.49, – N. 12, – P.408-417 (IF=1.055)
14. M.T. Zhybak, L.Y. Fayura, Y.R. Boretsky, M.V. Gonchar, A.A. Sibirny, E. Dempsey, A.P. F. Turner, Y.I. Korpan. amperometric l-arginine biosensor based on a novel recombinant arginine deiminase // Microchimica Acta. – 2017. – DOI 10.1007/s00604-017-2290-4 (IF=4.580)
15. Karkovska MI, Stasyuk N.E., Gayda G.Z., Smutok O.V., Gonchar M.V. Nanomaterials in the сonstruction of biosensors for biomedical applications // In the Book “Multifunctional nanomaterials for biology and medicine: molecular design, synthesis and application” (Ed. R.S. Stoika) - 2017. – P. 165-177
16. Stasyuk N., Gayda G., Zakalskiy A., Zakalska O., Fayura L., Vovk O., Stasyk O., Sibirny A., and Gonchar M. Recombinant forms of arginase and argininedeiminaseas catalytic components of argitest enzymatic kit for l-arginine analysis // Nauka innov. – 2017. - 13(4).- P. 64-72
17. Tsiko U., Yatsyshyn M., Kulik Y., Serkiz R., Pandyak N. Solid-phase synthesis of polyaniline and kaolin/polyaniline composite // Visnyk of the Lviv University. Series Chemistry.- 2017. - Issue 58. - P. 393-405

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Analytical Biotechnology

Research Grants

Current International Grants:

1. 2008-2009 STCU-4378 "Gene and Protein Engineering of Oxidoreductases for Construction of Bionanosized Objects of Analytical Importance".
2. 2008 NATO Linkag Grant PDD (CP)-(CPP.NUKR.CLG 982955) "Novel Electrochemical and Optical Nanostructured Sensors for Detection of Disease-Related Ions".
3. NATO Linkage Grant LST.NUKR.CLG 980621 "Novel Biological and Chemical Sensors for Formaldehyde Monitoring in Wasteswaters, Foodstuffs and Pharmaceuticals".
4. 2008-2010 NATO Linkage Grant PDD (CP)-(CPP.NUKR.CLG 982955) "Bio/Chemical Sensors for Detection of Toxins in Fish Meat”, STCU-project 4378 „Gene and Protein Engineering of Oxidoreductases for Construction of Bionanosized Objects of Analytical Importance".
5. 2008-2010 Ukrainian-Russian F28/454 "Structural and functional characterization of alcohol oxidase from thermotolerant methylotrophic yeast Hansenula polymorpha".
6. 2010 Ukrainian-izrailan M157 "Control of formaldehyde content in air by biosensor based on formaldehyde dehydrogenase and its removing by bioreactor with immobilized alcohol oxidase".
7. 2010-2011 CRDF UKB2-9044-LV-10 "Production of trial series and preparation of technical documentation for bioanalytical kit for enzymatic analysis of l-lactate in clinical diagnostics and beverage industries".
8. 2010-2011 CRDF UKB1-9048-KV-10 "Development of technology for production of recombinant human arginase as anticancer enzyme and element of biosensor for arginine".
9. 2010-2011 Polish-Ukrainian N 304 326136 "Mutants of nonconventional yeasts for efficient chromate bioremediation, recovery and industrial waste-water purification".
10. 2012-2015 NATO scientific project in the frame of the Program "Science for Peace and Security" SPS(NUKR)SFPP 984173 "Novel electrochemical Nano-Sensors for toxic ions Detection" (2012-2013).
11. 2013-2015 Polish-Ukrainian "Scientific integration of the Polish-Ukrainian borderland area in the field of monitoring and detoxification of harmful substances in environment".
12. 2023-2025 IMPRESS-U (project STCU-7113) "A Universal Biosensing Platform Amplifying Signals Produced by NAD+/NADH-Dependent Enzymes".
13. 2025-2027 Polish-Ukrainian "Physicochemical characterization of synthetic catalytic nanomaterials suitable for the construction of amperometric biosensors".

 

National scientific projects:

 

1. 2010-2012 Ukrainian-American CRDF Program.

Project 1. UKB2-9044-LV-10 "A new bioanalytical kit for enzymatic determination of L-lactate in clinical diagnostics and beverage production".

Project 2. UKB1-9048-KV-10 "Recombinant human arginase as a new antitumor drug" (in collaboration with the Department of Cell Signaling Mechanisms).

2. 2009-2012 Ukrainian-Russian project F28/454 "Structural and functional characterization of alcohol oxidase of thermotolerant methylotrophic yeast Hansenula polymorpha" was carried out within the framework of the program of the Foundation for Fundamental Research of the Ministry of Education and Science of Ukraine (now the State Committee of Ukraine for Science, Innovations and Informatization of the Ministry of Science of Ukraine).
3. 2003-2006 Targeted program "Physiological-biochemical and molecular-genetic foundations of the functioning of living systems and the development of principles for their management".

Topic: "Biotechnological production and functional characterization of proteins important for diagnostics and treatment".

4. 2003-2006 Comprehensive program of fundamental research "Research in the field of sensor systems and technologies".

Project 1. "Development of new biosensor and enzymatic systems for the analysis of glycerol in alcoholic beverages and the determination of triglycerides in biological fluids".

Project 2. "Design of enzyme elements of sensors based on oxidoreductases for monitoring the content of lactate and ethanol in biological fluids and fermentation cultures".

Project 3. "Design of yeast aminooxidase producers and development of biosensors for the analysis of biogenic amines in food products and human biological fluids".

5. 2007-2012 Comprehensive scientific and technical program "Sensor systems for medical-ecological and industrial-technological needs".

Project 1. "Development and manufacture of experimental samples of electrochemical biosensors for the analysis of formaldehyde in food products, pharmaceuticals and the environment. Development of a formaldehyde analyzer based on amperometric electrodes".

Project 2. "Portable analyzer based on amperometric enzyme biosensors for quality control of beverages in winemaking".

6. 2013-2014 Comprehensive scientific and technical program of the NAS of Ukraine "Sensor devices for medical-ecological and industrial-technological needs: metrological support and experimental operation".

Project 1. "Development of bio/chemosensor analyzers for determining the content of formaldehyde, L- and D-lactate, their metrological evaluation and experimental testing. Development of an amperometric analyzer for determining the content of formaldehyde, L- and D-lactate based on the "FORMATEST-2010" device.

Project 2. "Development and experimental operation of a portable analyzer based on amperometric enzyme biosensors for quality control of beverages in winemaking. Development of biosensor methods for the analysis of arginine in wine using arginine-hydrolyzing enzymes".

7. 2010-2012 Comprehensive scientific research program "Biomass as a fuel raw material (biofuel): "Thermotolerant strains of Saccharomyces yeast as producers of bioethanol at elevated temperatures for the alcohol industry".

8. 2013-2014 Targeted comprehensive scientific research program of the NAS of Ukraine "Biological resources - new technologies of bioenergy conversion".

Project 1. "Selection and creation of thermotolerant strains of Saccharomyces – producers of bioethanol using mutagenesis".

9. 2018-2022 Comprehensive scientific and technical program of the NAS of Ukraine "Smart" sensor devices of a new generation based on modern materials and technologies".

Project 1. Scientific and technical project "Development and optimization of enzymatic electrochemical biosensors based on new functional nanomaterials for arginine determination and their application for quality control of fruit and berry juices and hygiene products" No. 10/3-2018.

Project 2. Scientific and technical project "Creation of smart sensor devices for the analysis of creatinine and ammonium ions based on new nanocomposite films".

10. 2018-2019 Scientific and technical project "Design of thermotolerant yeast strains with improved parameters of substrate bioconversion (xylose, glucose, glycerol) to ethanol".
11. 2018 Fundamental research project "Investigation of new composite materials with ion-synthesized metal nanoparticles for sensors".
12. 2018-2020 Fundamental research project "Optimization of enzyme immobilization conditions on nanoparticles in polymer matrices to improve the operational parameters of lactate-selective biosensors".
13. 2018-2019 Applied research project "Creation of a non-invasive integrated approach to assess the adequacy of physical activity in physical rehabilitation and sports".
14. 2019-2020 Fundamental research project "New nanocomposite polymer matrices for the construction of prototypes of bioreactors and biosensors for the removal and monitoring of xenoestrogens".
15. 2020-2021 Ukrainian-Lithuanian project "Development of biosensors and biofuel cells using oxidoreductases" M/20-2020.
16. 2020-2023 Project of the National Fund of Ukraine "Creation of new nanozymes as catalytic elements for enzymatic".

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Analytical Biotechnology

Personnel:

• Mykhailo Gonchar – Head of the Department, D. Sci. (Biol.), Professor, Corresponding Member of NAS of Ukraine;
• Nataliya Stasyuk – Senior Researcher, D. Sci. (Biol.);
• Halyna Gayda – Senior Researcher, Ph.D. (Chem.), Senior Researcher;
• Andriy Zakalskyy – Researcher, PhD;
• Tetiana Prokopiv – Researcher, PhD;
• Olha Demkiv – Researcher, PhD;
• Oksana Zakalska – Lead Engineer;
• Olga Smyk – Engineer;
• Mariya Gorecha – Engineer;
• Roman Serkiz – Engineer.