2015

1. Wojnarowska R., Polit J., Broda D., Gonchar M., Sheregii E.M. Surface enhanced Raman scattering as a probe of cholesterol oxidase enzyme // Appl. Physics Letters. – 2015. – V. 106, 103701. doi: 10.1063/1.4914499 (IF=3.515)
2. Stasyk O., Boretsky Y., Gonchar M., Sibirny A. Recombinant arginine-degrading enzymes in metabolic anticancer therapy and biosensorics // Cell Biol. Intern. - 2015. - V. 39, N 3. – P. 246-252 (IF=1.640)
3. Stasyuk N.Ya., Gayda G.Z., Serkiz R.Ja., Gonchar M.V. Cell Imaging with Fluorescent Bi-Metallic Nanoparticles // Journal of Advances in Chemistry. - 2015. - V. 11, No. 4. – P. 3498–3510 (IF=1.415)
4. Sibirnyj W., Grabek-Lejko D., Gonchar M. The use of enzymes for ethanol, methanol and formaldehyde determination in food products // Journal of Microbiology, Biotechnology and Food Sciences. – 2015. – V. 4, No 5. – P. 393-397.

2016

1. Karkovska M., Smutok O., Gonchar M. Laboratory prototype of bioreactor for oxidation of toxic D-lactate using yeast cells overproducing D-lactate-cytochrome c oxidoreductase // BioMed Research Intern. – 2016. – P. 1-5. (IF=2.134)
2. Lesanavičius M., Smutok O., Valiauga B., Marozienė A., Gonchar M., Krikštopaitis K., Čėnas N. Kinetic properties of flavocytochrome b₂ from Hansenula polymorpha // Chemija. – 2016. - V. 2, N 2. - P. 23-127. (IF=0.472)
3. Stasyuk N., Gayda G., Gonchar M. Novel arginine deiminase-based method to assay L-Arginine in beverages // Food Chemistry. – 2016. – V. 201. – P. 320-326 (IF=4.052)
4. Stasyuk N., Gayda G., Yepremyan H., Stepien A., Gonchar M. Fluorometric enzymatic assay of L-arginine // Spectrochim. Acta Part A: Molecular and Biomolecular Spectroscopy. - 2017. - V. 170. – P. 184–190. (available online 11 July 2016) (IF=2.653)
5. Stasyuk N., Gayda G., Klepach H., Gonchar M. Nanoparticles of noble metals as effective platforms for the fabrication of amperometric biosensor on hydrogen peroxide // Sensor Letters. – 2016. – V. 14, N 11-12. – P. 1–9 (IF=0.320)
6. Stasyuk N., Synenka M., Gayda G., Smutok O., Gonchar M. Arginase-based amperometric biosensor for manganese ions analysis. // “Eureka. Life science”. – 2016. – N 1. – P. 22-28
7. Stasyuk N., Gayda G., Serkiz R., Gonchar M.. The “green” synthesis of gold nanoparticles by the yeast Hansenula рolymorpha // Вісник Львівського університету. – Вип.73. – С. 96-102
8. L.V. Kobrina, V.V. Boiko, T.V. Dmitrieva, V.V. Bortnitsky, T.M. Prokopiv, S.V. Riabov, M.V. Gonchar. Influence of physical factors and cultures of microorganisms on segmented polyurethane’s degradation // Polimer. Journal. - 2016. – V.38, N3. – P. 236-243
9. O. Demkiv, S. Banah, H. Klepach, G. Gayda, O. Smutok, M. Gonchar. Fungal oxidases: screening of strains and perspectives of bioanalytical application // Visnyk of the Lviv University. Series Biology. 2016. Issue 73. P. 55–64
10. Demkiv O., Banah S., Prytula S., Gayda G., Gonchar M. Screening of fungal strains – producers of laccase // ScienceRise:Biological Science. - 2016. - № 3 (3). - P. 66-69
11. Karkovska M., Smutok O., Gonchar M. Usage of flavocytochromeb2 immobilized onto magnetic microparticles in multiple enzymatic analysis of L-lactate // Visnyk of the Lviv University. Series Biology. 2016. Issue 72. P. 25-32

2017

1. Demkiv O., Smutok O., Gonchar M., Nisnevitch M. Reagentless amperometric formaldehyde-selective chemosensor based on using platinized gold electrode // Materials. – 2017. - V.10, N.5 - E503 (1-11) doi:10.3390/ma10050503 (IF=2.654)
2. Gayda G., Gonchar M., Sibirny A. Yeast-based systems for environmental control // In the Book "Biotechnology of Yeasts and Filamentous Fungi" (Ed. A.A. Sibirny). – Springer, 2017. – P. 373-386
3. Gayda G.Z., Stasyuk N.Ye, Serkiz R.Ja, Gonchar M.V. Fluorescent nanoparticles of noble metals for cell imaging // В книзі: “Багатофункціональні наноматеріали для біології і медицини: молекулярний дизайн, синтез і застосування” (ред. Р.С. Стойка) – 2017. – C. 156-165
4. Gonchar M., Smutok O., Karkovska M., Stasyuk N. Yeast-based biosensors for clinical diagnostics and food control // In the Book "Biotechnology of Yeasts and Filamentous Fungi" (Ed. A.A. Sibirny). - Springer, 2017. – P. 392-400
5. Kavetskyy T., Smutok O., Gonchar M., Demkiv O., Klepach H., Kukhazh Y., Šauša O., Petkova T., Boev V., Ilcheva V., Petkov P., Stepanov A.L. Laccase-containing ureasil-polymer composite as the sensing layer of an amperometric biosensor // Journal of Applied Polymer Science. – 2017. – V.134 (36). – P. 45278-45285 (IF=1.866)
6. Kavetskyy T., Šauša O., Čechová K., Švajdlenková H., Mat’ko I., Petkova T., Boev V., Ilcheva V., Smutok O., Kukhazh Y. and Gonchar M. Network Properties of Ureasil-Based Polymer Matrixes for Construction of Amperometric Biosensors as Probed by PALS and Swelling Experiments // Acta Physica Polonica A. - 2017. - V. 132, N 5. - P. 1515-1519 (IF=0.469)
7. Kovalyshyn Y., Konovska M., Milanese C., Saldan I., Serkiz R., Pereviznyk O., Reshetnyak O., Kuntyi O. Electrochemical properties of the composites synthesized from polyaniline and modified MWCNT // Chem. Chem. Technol., – 2017, – V. 11, N. 3, – P. 261-269 (in Scopus)
8. Smutok O., Karkovska M., Serkiz Ya., Vus B., Čenas N., Gonchar M. A Novel Mediatorless Biosensor Based On Flavocytochrome b₂ Immobilized Onto Gold Nanoclusters For Non-invasive L-lactate Analysis Of Human Liquids // Sensor & Actuators B. – 2017. - V. 250. - P. 469-475, DOI: 10.1016/j.snb.2017.04.192 (IF=5.401)
9. Stasyuk N., Gayda G., Serkiz R., Gonchar M. The “green” synthesis of gold nanoparticles by the yeast Hansenula рolymorpha // Вісник Львівського університету. Серія біологічна. - 2016. – Вип. 73. - С. 96-102
10. Stasyuk N., Gayda G., Yepremyan H., Gonchar M. Fluorometric enzymatic assay of L-arginine // Spectrochimica Acta. Part A. – 2017. – V. 170. – P. 184-190 (IF=2.653)
11. Stasyuk, N., Gayda, G., Klepach, H., Semashko, T., Gonchar, M., 2017. Nanoparticles of noble metals for enzymatic sensors: an amperometric glucose biosensor for wine analysis // Sensor Letters. – 2017 – V.15, N8. – P. 647-654 (IF=0.558)
12. Stepien A.E.,  Zebrowski J., Piszczyk Ł., Boyko V.V.,   Riabov S.V., Dmitrieva T.,  Bortnitskiy V.I.,  Gonchar M., Wojnarowska-Nowak R., Ryszkowska J. Assessment of the impact of bacteria Pseudomonas denitrificans, Pseudomonas fluorescens, Bacillus subtilis and yeast Yarrowia lipolytica on commercial poly(ether urethanes) // Polymer Testing. – 2017. – V.63. – P.484-493. (IF=2.464)
13. Toporovska L.R., Hryzak A.M., Turko B.I., Rudyk V.P., Tsybulskyi V.S., Serkiz R.Y.. Photocatalytic properties of zinc oxide nanorods grown by different methods // Optical and Quantum Electronics, – 2017, – Vol.49, – N. 12, – P.408-417 (IF=1.055)
14. M.T. Zhybak, L.Y. Fayura, Y.R. Boretsky, M.V. Gonchar, A.A. Sibirny, E. Dempsey, A.P. F. Turner, Y.I. Korpan. amperometric l-arginine biosensor based on a novel recombinant arginine deiminase // Microchimica Acta. – 2017. – DOI 10.1007/s00604-017-2290-4 (IF=4.580)
15. Karkovska MI, Stasyuk N.E., Gayda G.Z., Smutok O.V., Gonchar M.V. Nanomaterials in the сonstruction of biosensors for biomedical applications // In the Book “Multifunctional nanomaterials for biology and medicine: molecular design, synthesis and application” (Ed. R.S. Stoika) - 2017. – P. 165-177
16. Stasyuk N., Gayda G., Zakalskiy A., Zakalska O., Fayura L., Vovk O., Stasyk O., Sibirny A., and Gonchar M. Recombinant forms of arginase and argininedeiminaseas catalytic components of argitest enzymatic kit for l-arginine analysis // Nauka innov. – 2017. - 13(4).- P. 64-72
17. Tsiko U., Yatsyshyn M., Kulik Y., Serkiz R., Pandyak N. Solid-phase synthesis of polyaniline and kaolin/polyaniline composite // Visnyk of the Lviv University. Series Chemistry.- 2017. - Issue 58. - P. 393-405

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Analytical Biotechnology

Research Grants

Current International Grants:

1. 2008-2009 STCU-4378 "Gene and Protein Engineering of Oxidoreductases for Construction of Bionanosized Objects of Analytical Importance".


2. 2008 NATO Linkag Grant PDD (CP)-(CPP.NUKR.CLG 982955) "Novel Electrochemical and Optical Nanostructured Sensors for Detection of Disease-Related Ions".


3. NATO Linkage Grant LST.NUKR.CLG 980621 "Novel Biological and Chemical Sensors for Formaldehyde Monitoring in Wasteswaters, Foodstuffs and Pharmaceuticals".


4. 2008-2010 NATO Linkage Grant PDD (CP)-(CPP.NUKR.CLG 982955) "Bio/Chemical Sensors for Detection of Toxins in Fish Meat”, STCU-project 4378 „Gene and Protein Engineering of Oxidoreductases for Construction of Bionanosized Objects of Analytical Importance".


5. 2008-2010 Ukrainian-Russian F28/454 "Structural and functional characterization of alcohol oxidase from thermotolerant methylotrophic yeast Hansenula polymorpha".


6. 2010 Ukrainian-izrailan M157 "Control of formaldehyde content in air by biosensor based on formaldehyde dehydrogenase and its removing by bioreactor with immobilized alcohol oxidase".


7. 2010-2011 CRDF UKB2-9044-LV-10 "Production of trial series and preparation of technical documentation for bioanalytical kit for enzymatic analysis of l-lactate in clinical diagnostics and beverage industries".


8. 2010-2011 CRDF UKB1-9048-KV-10 "Development of technology for production of recombinant human arginase as anticancer enzyme and element of biosensor for arginine".


9. 2010-2011 Polish-Ukrainian N 304 326136 "Mutants of nonconventional yeasts for efficient chromate bioremediation, recovery and industrial waste-water purification".


10. 2012-2015 NATO scientific project in the frame of the Program "Science for Peace and Security" SPS(NUKR)SFPP 984173 "Novel electrochemical Nano-Sensors for toxic ions Detection" (2012-2013).


11. 2013-2015 Polish-Ukrainian "Scientific integration of the Polish-Ukrainian borderland area in the field of monitoring and detoxification of harmful substances in environment".

National scientific projects:

1. 2010-2012 CRDF.



Project 1. UKB2-9044-LV-10 "Production of trial series and preparation of technical documentation for bioanalytical kit for enzymatic analysis of l-lactate in clinical diagnostics and beverage industries".




Project 2. CRDF UKB1-9048-KV-10 "Development of technology for production of recombinant human arginase as anticancer enzyme and element of biosensor for arginine".



2. 2009-2012 Ukrainian-Russian F28/454 "Structural and functional characterization of alcohol oxidase from thermotolerant methylotrophic yeast Hansenula polymorpha".


3. 2003-2006 Program "Physiological, biochemical and molecular genetic bases of living systemsʼ functioning and development of principles for their management".



Theme: "Biotechnological production and functional characteristics of the proteins, important for diagnostics and treatment".




Task: "Development of the effective systems for purification of oxido-reductases from microbial cells".



4. 2003-2006 Program "Investigations in the field of sensorsʼ systems and technologies".



Project 1. "Construction of the enzymesʼ elements of sensors on the base of oxido-reductases to control the content of lactate and ethanol in biological fluids and fermentation broths".




Project 2. "Development of the new biosensorsʼ and enzymatic systems for analysis of glycerol in alcoholic beverages and triglycerides in biological fluids".




Project 3. "Construction of amine oxidase over-producing yeast strains and development of biosensor for assay of natural amines in food and in human biological fluids".



5. 2007-2012 Program "Sensorsʼ systems for medical-ecological and industrial-technological requirements".



Project 1. "Development and production of the experimental samples of electrochemical biosensors for assay of formaldehyde in foods".




Project 2. "Portative analyzer based on amperometric enzyme biosensors for the quality control of the beverages in wine-making".



6. 2010-2012 Program "Biomass as a fuel source (biofuel)".



Project 1. "Screening and selection of the thermotolerant Saccharomycetes strains with enhanced ethanol-producing activity".



7. 2013-2014 Program "Sensorsʼ devices for medical-ecological and industrial-technological requirements: meteorological support and research exploitation".



Project 1. "The development of bio / chemosensor analyzers for determination of formaldehyde, L- and D-lactate, their metrological evaluation and experemental testing. The construction of amperometric analyzer for assay of formaldehyde,L- and D-lactate based on the device "FORMATEST-2010".




Project 2. "The development and trial exploitation of a portative analyzer based on amperometric enzyme biosensors for quality control of beverages in winery. Development of biosensor methods of arginine analysis in wine by using arginine-hydrolyzing recombinant enzymes".



8. 2013-2014 Program "Biological resources novel technologies of bioenergy conversion".



Project 1. "Screening and selection of the thermotolerant Saccharomycetes strains – overproducers of bioethanol using mutagenesis".

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Analytical Biotechnology

Personnel:

• Gonchar Mykhailo – Prof., Head of the Department
• Gayda Halyna – PhD
• Smutok Oleh – PhD
• Zakalskyy Andriy – PhD
• Stasyuk Nataliya – PhD
• Prokopiv Tetiana – PhD
• Demkiv Olha – PhD
• Kutsiaba Vasyl – PhD
• Klepach (Pavlishko) Galyna – PhD
• Nechay Galyna – PhD
• Zakalska Oksana
• Lavryk (Karkovska) Mariya
• Ivash Mariya
• Synenka Mariya
• Serkiz Roman