Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Cell Signaling

The main scientific interests of the Department are:

• Search for new approaches to metabolic anticancer therapy, in particular, based on artificial starvation for individual amino acids and combined approaches;


• Research on natural analogues of amino acids as potential antitumor and antimicrobial agents;


• Development of new model of Parkinson's disease based on methylotrophic yeasts;


• Designing stable hybridoma clones and monoclonal antibodies against protein-antigens of biotechnological and medical importance to develop new diagnostic systems.

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Cell Signaling

Publications

2008

1. Stasyk O.G., Maidan M., Stasyk O.V., Van Dijck P., Thevelein J.M., Sibirny A.A. Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha // Eukaryotic Cell. -2008.- V.7, № 4. – P.735-746.
2. Ishchuk O.P., Voronovsky A.Y., Stasyk O.V., Gayda G.Z., Gonchar M.V., Abbas C.A., Sibirny A.A. Improvement of xylose high-temperature fermentation in Hansenula polymorpha due to overexpression of the PDC1 gene coding for pyruvate decarboxylase // FEMS Yeast Research. – 2008. – V.8, № 7. – P.1164-1174.
3. Sybirna N., Brodiak I., Ferents I., Barska M. The effect of L-arginine on the oxidative modification of plasma fibrinogen under experimental diabetes mellitus in rats // Annal. Univers. Mariae Curie-Sklodovska, sectio AA Biologia – 2008. – V. IX,– P. 115-118.

2009

1. Havrylov S., Rzhepetskyy Yu., Malinowska A., Drobot L., Rendowicz M.J. Proteins recruited by SH3 domains of Ruk/CIN85 adaptor identified by LC-MS/MS // Proteome Science.-2009.-Vol. 7, N21.-P. 1-19.
2. O.I. Basaraba, M.L. Barska, Ya.P. Bobak, H.Yu. Shuvayeva, S.M. Marchenko, V. R. Savran, N.A. Volodko, V.L. Buchman, L.B. Drobot. Expression of (RUK/CIN85)/(CD2AP/CMS) family adapter proteins in human breast adenocarcinoma // Clin. and Experim. Potol.-2009- V. 9, № 4. – P. 125-130.
3. Rzhepetskyy Y.А., Svinchuk V.V., Buchman V.L.,Drobot L.B. Comparative analysis of Ruk/CIN85 phosphorylation in rat pheochromocytome cell line PC12 under growth factors stimulation// Clinical and eksperimental pathology. 2009.- V. VIII, №2.- p. 64-67.
4. Brodyak I.V., Zdioruk N.І., Barska M.L., Vovk O.І., Sybirna N.O. Lectincytochemical analysis of mononuclear leukocyte plasmatic membrane sialocontaining glycoprotein of peripheral blood under type 1 diabetes mellitus // Med.Chim. – 2009. – № 4. – P. 76–79 (Ukrainian).
5. Zdioruk M.I., Barska M.L., Brodyak I.V., Vovk O.I. Urbanovych A.M., Sybirna N.O. Influence of wortmannin on aggregation ability of neutrophylic granulocytes under type 1 diabetes mellitus / Studia Biologica.– 2009. –V.3, №2. – P. 133–140 (Ukrainian).

2010

1. Bobak Y.P., Vynnytska B.O., Kurlishchuk Y.V., Sibirny A.A., Stasyk O.V. Cancer cell sensitivity to arginine deprivation in vitro is not determined by endogenous levels of arginine metabolic enzymes // Cell Biol Int.-2010.-V.34 (11).-P.1085–1089.
2. Stadnyk V.V., Izyumovaa L.A., Rzhepetskiy Y.A., Mayor C.Y., Vlizlo V.V. The Antisense Oligonucleotides Decrease Expression of the Cellular Prion // Letters in Drug Design & Discovery.- 2010,-V.7.- P.23-26.
3. Jóźwiak J., Rzhepetskiy Y., Sobczak M., Kocik E., Skórzewski R., Kłopocka W., Rędowicz M.J. Amebin a novel Amoeba Proteus actin-bundling protein inhibits actomyosin ATPase activity and is essential for Amoeba migration // Archives of Biochemistry and Biophysics.- 2010, (published ahead of print).

Tutorials and methodical editions:

1. Chemistry of the Protein: texbook: [for university students] / [ N.О. Sybyrna, М.V. Gonchar, І.V. Brodyak, О.G. Stasyk, М.L. Barska] edited by Prof. N.О. Sybyrna. - Lviv: Ivan Franko Lviv national University. 2010 - pp.393 . Labeled by MES of Ukraine.

2011

1. Vynnytska B.O., Mayevska O.M., Kurlishchuk Y.V., Bobak Y.P., Stasyk O.V. Canavanine augments proapoptotic effects of arginine deprivation in cultured human cancer cells // Anticancer Drugs. – 2011. – V.22 (2). – P. 148 – 157. (IF=2.407) (Scopus)
2. Jozwiak J, Rzhepetskyy Y, Sobczak M, Kocik E, Skorzewski R, Klopocka W, Redowicz MJ. C-terminal fragment of amebin promotes actin filament bundling, inhibits acto-myosin ATPase activity and is essential for amoeba migration // Arch. Biochem. Biophys. – 2011. – V. 506(1). – P. 99-108. (IF=2.935) (Scopus)
3. Nazarko V.Y., Nazarko T.Y., Farre J.C., Stasyk O.V., Warnecke D., Ulaszewski S., Cregg J.M., Sibirny A.A., Subramani S. Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris // Autophagy. – 2011. – V. 7(4). – P. 375-385. (IF=7.453) (Scopus)
4. Sybirna N., Brodyak I., Zdioruk M., Barska M. Sialic acid-containing glycoproteins are the marker molecules that determine the leukocyte functional state under diabetes mellitus // Sepsis. 2011. – V. 4(1) – P. 47-55. (Google Scholar)
5. Стасюк Н.Є., Карпʼяк В.В., Гайда Г.З., Гайда А.В., Стасик О.В., Гончар М.В. Синтез афінних сорбентів для виділення рекомбінантної аргінази і людини із генетично модифікованих дріжджів Нansenula polymorpha //Праці НТШ, Хем. Біохем. – 2011. – Т. 28. – С.139-149. (Google Scholar)
6. O.I. Chen, L.S. Lyniv, N.I. Igumentseva, M.L. Barska, N.O. Effect of nitric oxide donor on viability of human leukemic cells upon arginine deprivation // Studia Biologica. – 2011. – V. 5, N 2. – P. 17–28. (Google Scholar)
7. Сибірна Н.О., Здіорук М.І., Бродяк І.В., Барська М.Л., Вовк О.І. Активація фосфатидилінозитол-3′-кіназного шляху лектиніндукованим сигналом через сіаловмісні глікопротеїни мембран лейкоцитів при цукровому діабеті 1-го типу // Укр. біохім. Журнал. – 2011. – Т. 83. - № 5. – С. 22-31. (Google Scholar)
8. Vovk O.I., Rzhepetskyy Y.A., Pereverzeva H.G., Stasyk O.V. Expression of recombinant human argininosuccinate synthetase in Escherichia coli // Біологічні Студії / Studia Biologica. – 2011. – Том 5. - №3 - С. 19–28. (Google Scholar)
9. Стасюк Н., Гайда Г., Гайда А., Стасик О., Карпʼяк В., Гончар М. Синтез афінних сорбентів для очищення аргінази І людини із рекомбінантних дріжджів Нansenula polymorpha // Праці НТШ, Хем. Біохем. – 2011. – Т.28. – С.139-149. (Google Scholar)
10. Стасик О. Г., Стасик О. В. Селективна деградація пероксисом у дріжджів Hansenula polymorpha потребує стеролглюкозилтрансферазу Atg26 // Біологічні студії. – 2011. – Т.5, №2. – С. 93-104. (Google Scholar)
11. Ю.В. Курліщук, Б.О. Винницька-Мироновська, Я.П. Бобак, О.В. Стасик (2011) Вплив метаболітів аргініну на життєздатність людських пухлинних клітин за умов дефіциту цієї амінокислоти in vitro. Біологічні студії. – 2011. - Т.5, №2. - С. 5-16. (Google Scholar)

2012

1. Vynnytska-Myronovska B., Bobak Y., Garbe Y., Dittfeld C., Stasyk O., Kunz-Schughart L.A. Single amino acid arginine starvation efficiently sensitizes cancer cells to canavanine treatment and irradiation // Int. J. Cancer. – 2012. – V. 130, № 9. – P. 2164 – 2175 (IF=6.198). (Scopus)
2. Zakalskiy AE, Zakalska OM, Rzhepetskyy YA, Potocka N, Stasyk OV, Horak D, Gonchar MV. Overexpression of (His)(6)-tagged human arginase I in Saccharomyces cerevisiae and enzyme purification using metal affinity chromatography // Protein Expr Purif. -2012.- 81(1). –P. 63-68. (IF=1.429) (Scopus)
3. Samoylenko A., Vynnytska-Myronovska B., Byts N., Kozlova N., Basaraba O., Pasichnyk H., Palyvoda K., Bobak Ya., Barska M., Mayevska O., Rzhepetskyy Y., Shuvayeva H., Lyzogubov V., Usenko V., Savran V., Volodko N., Buchman V.L., Kietzmann T., Drobot L. Increased levels of the HER1 adaptor protein Rukl/CIN85 contribute to breast cancer malignancy // Carcinogenesis. - 2012.- Vol. 33, №10.-P. 1976-1984 (IF=5.635) (Scopus)
4. Сибірна Н.О., Бродяк І.В., Барська М.Л., Вовк О.І. Участь ензиму фосфатидилінозитол-3ʼ-кінази у процесах сприйняття і передачі сигналів через галактозилумісні глікопротеїнові рецептори сегментоядерних лейкоцитів при цукровому діабеті 1 типу // Фізіологічний журнал. – 2012. - T. 58, №6. - С. 9-22. (Google Scholar)
5. O. Chen, M. Barska, N. Sybirna, O. Stasyk. Development and evaluation of HPLC method for arginine assay in biological samples // Вісник Львівського університету. Серія біологічна. – 2012. – Том 60. –С. 73–82. (Google Scholar)

2013

1. Krasovska O.S., Stasyk O.V., Sibirny A.A. Stable overproducer of hepatitis B surface antigen in the methylotrophic yeast Hansenula polymorpha due to multiple integration of heterologous auxotrophic selective markers and defect in peroxisome biogenesis // Appl. Microbiol. Biotechnol. – 2013. – Vol. 97, No. 23. – P. 9969-9979 (ІF=3.811) (Scopus)
2. Vynnytska-Myronovska B., Kurlishchuk Y., Bobak Y., Dittfeld C., Kunz-Schughart L.A., Stasyk O. Three–dimensional environment renders cancer cells profoundly less susceptible to a single amino acid starvation // Amino Acids. – 2013. – V. 45, № 5. – P. 1221-1230 (IF=3.653) (Scopus)
3. Chen O., Kavalets B., Barska M., Lyniv L., Vovk O., Sybirna N. Stasyk O. Effect of combinational arginase and canavanine treatment on normal human peripheral blood lymphocytes in vitro // Curr. Issues Pharm. Med. Sci. – 2013 - Vol. 26, No. 4, Pages 385–389. (Scopus)
4. Sybirna N.O., Brodyak I.V., Barska M.L., Vovk O.I. Participation of Phosphatidylinositol-3ʼ-kinase in Perception and Signal Transduction through Galactosyl-Containing Glycoprotein Receptors of Segmented Leukocytes under Type 1 Diabetes Mellitus // International Journal of Physiology and Pathophysiology. – 2013– V.4, №3. – P. 213-230. (IF=0.150) (Google Scholar)

2014

1. Petryk N., Zhou Y.F., Sybirna K., Mucchielli M.H., Guiard B., Bao W.G., Stasyk O.V., Stasyk O.G., Krasovska O.S., Budin K., Reymond N., Imbeaud S., Coudouel S., Delacroix H., Sibirny A., Bolotin-Fukuhara M. Functional study of the Hap4-like genes suggests that the key regulators of carbon metabolism HAP4 and oxidative stress response YAP1 in yeast diverged from a common ancestor. PLoS One. 2014 Dec 5;9(12):e112263. doi: 10.1371/journal.pone.0112263. eCollection 2014. (IF=3.234) (Scopus)
2. Shuvayeva Galyna, Bobak Yaroslav, Igumentseva Natalia, Titone Rossella, Morani Federica, Stasyk Oleh, Isidoro Ciro. Single Amino Acid Arginine Deprivation Triggers Prosurvival Autophagic Response in Ovarian Carcinoma SKOV3 // BioMed Research International. Volume 2014 , Article ID 505041, 10 pages, (IF=1.579) (Scopus)
3. Mathias Stiller, Martyna Molak, Stefan Prost, Gernot Rabeder, Gennady Baryshnikov, Wilfried Rosendahl, Susanne Münzel, Hervé Bocherens, Aurora Grandal-dʼAnglade, Brigitte Hilpert, Mietje Germonpré, Oleh Stasyk, Ron Pinhasi, Andrea Tintori, Nadin Rohland, Elmira Mohandesan, Simon Y.W. Ho, Michael Hofreiter, Michael Knapp Mitochondrial DNA diversity and evolution of the Pleistocene cave bear complex // Quaternary International. – 2014. – 339–340:224-231 (IF=2.062) (Scopus)
4. Vynnytska-Myronovska B.O., Bobak Y.P., Pasichnyk G.V., Igumentseva N.I., Samoylenko A.A., Drobot L.B.. Multiple molecular forms of adaptor protein Ruk/CIN85 specifically associate with different subcellular compartments in human breast adenocarcinoma MCF-7 cells. Ukr Biochem J. 2014 Sep-Oct;86(5):102-10. (Google Scholar)
5. Denega I.O., Klymyshyn N.I., Sybirna N.O., Stasyk О.V., Stasyk О.G.. Modeling of molecular processes underlying Parkinsonʼs disease in cells of methylotrophic yeast Hansenula polymorpha // Біологічні студії/Studia Biologica. – 2014. – Т. 8, № 12. – C. 5–16. (Google Scholar)

2015

1. Stasyk O.V., Boretsky Y.R., Gonchar M.V. and Sibirny A.A. Recombinant arginine-degrading enzymes in metabolic anticancer therapy and bioanalytics. // Cell Biology International. – 2015 – Vol. 39, No.3 – P. 246–252. (IF=1.933) (Scopus)
2. Pavlyk Yu., Rzhepetskyy Yu., Jagielski A., Drozak J., Wasik A., Pereverzieva G., Olchowik M., Kunz‑Schugart L., Stasyk O., Redowicz MJ. Arginine deprivation affects glioblastoma cell adhesion, invasiveness and actin cytoskeleton organization by impairment of β‑actin arginylation // Amino Acids. – 2015. – V.47. P. 199–212 (IF=3.293) (Scopus)
3. Karolczak J1., Pavlyk I., Majewski Ł., Sobczak M., Niewiadomski P., Rzhepetskyy Y., Sikorska A., Nowak N., Pomorski P., Prószyński T., Ehler E., Rędowicz M.J. Involvement of unconventional myosin VI in myoblast function and myotube formation. Histochem Cell Biol. 2015 Jul;144(1):21-38. (IF=3.054) (Scopus)
4. Fedorovych D., Boretsky Y., Bobak Ya., Prokopiv T., Sybirny A. Putative ferroxidases in the flavinogenic yeast Pichia guilliermondii are regulated by iron acquisition. // Tsitologiia i genetika (Cytology and Genetics). – 2015. – Vol.49, No.5 – P. 13-19. (IF=0.379) (Google Scholar)