2015

Tomin A. Dumych T., Tolstyak Y., Kril I., Mahorivska I., Bila E., Stoika R., Herrmann M., Kit Y., Bilyy R. Desialylation of dying cells with catalytically active antibodies possessing sialidase activity facilitates their clearance by human macrophages. // Clin. Exp. Immunol. – 2015; 179:17-23. (IF=3.278)
Kit Y. Bilyy R, Korniy N, Tomin A, Chop'yak V, Tolstyak Y, Antonyuk V, Stoika R.Two-step chromatography purification of IgGs possessing sialidase activity from human blood serum. // Biomed. Chromatogr. 2015; 29(3):328-332. (IF=1.723)
Berdyshev A.G., Kosiakova H.V., Onopchenko O.V., Panchuk R.R., Stoika R.S., Hula N.M., N-Stearoylethanolamine suppresses the pro-inflammatory cytokines production by inhibition of NF-κB translocation. // Prostaglandins Other Lipid Mediat. – 2015. 121 (Pt A): 91-6. (IF=2.377)
Hor'ak D., Plichta Z., Starykovych M,. Myronovskij S., Kit Y., Chopyak V, Stoika R. Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus // RCS Advances. 2015, 5, 63050–63055. (IF=3.840)
Panchuk R.R., Prylutska S.V., Chumak V.V., Skorokhyd N.R., Lehka L.V., Evstigneev M.P., Prylutskyy Yu.I., Berger W., Heffeter P., Scharff P., Ritter U., Stoika R.S. Application of C60 fullerene-doxorubicin complex for tumor cell treatment in vitro and in vivo. // J. Biomed. Nanotechnol. – 2015. – V. 11, N.7. – P. 1139-1152. (IF=5.338)
Antonyuk V., Grama S., Plichta Z., Magorivska I., Horak D., Stoika R. Use of specific polysaccharide-immobilized monodisperse poly(glycidyl methacrylate) core-silica shell microspheres for affinity purification of lectins. // Biomed. Chromatography. – 2015. - V. 29, Issue 5. Р. 783 – 787. (IF=1.662)
Falfushynska H., Gnatyshyna L., Fedoruk O., Mitina N., Zaichenko A., Stoliar O., Stoika R. Hepatic metallothioneins in molecular responses to cobalt, zinc, and their nanoscale polymeric composites in frog Rana ridibunda. // Comp Biochem Physiol C Toxicol Pharmacol. 2015 Jun-Jul; 172-173: Р.45-56. (IF=2.301)
Tsivinska M.V., Panchak L.V., Stoika R.S., Antonyuk V.O. Isolation, characteristics, and antioxidant activity of low molecular compounds of fruit bodies Lactarius pergamenus (Fr.) Fr mushrooms. // Journal of Advances in Biology. - 2015- V 6, No. 3 p. 1023- 1035. (IF=1.561)
Buko V., Belonovskaya E., Naruta E., Lukivskaya O., Kanyuka O., Zhuk O., Kranc R., Stoika R., Sybirna N. Pituitary tumor transforming gene as a novel regulatory factor of liver fibrosis. // Life Sciences. 2015 Jul 1; 132: 34-40. (IF=2.702)
Tanasienko I.V,, Yemets A.I., Finiuk N.S., Stoika R.R., Blume Y.B. DMAEM-based cationic polymers as novel carriers for DNA delivery into cells. // Cell Biol Int. 2015 Mar; 39(3): Р.243-245. (IF=1.933)
Stoika R.S., Klyuchivska O.Yu., Biłoklicka G.F., Hodovana O.I. , Godovanyi O.V. Evaluation of individual biocompatibility of osteoplastic materials used in periodontal surgery: novel approach of ex vivo biotesting of fibroblast-like cells of gums bioptate of patients // Stomatologia-Wspolczesna – 2015; V22. N4. P. 10-19
Turcheniuk K., Turcheniuk V., Hage C.H., Dumych T., Bilyy R., Bouckaert J., Héliot L., Zaitsev V., Boukherroub R., Szunerits S. Highly effective photodynamic inactivation of E.coli using gold nanorod/SiO2 core-shell nanostructures with embedded verteporfin. // Chem Commun (Camb). 2015 Nov 3;51(91):16365-16368. (IF=6.834)
Yan X., Sivignon A., Alcouffe P., Burdin B., Favre-Bonté S., Bilyy R., Barnich N., Fleury E., Ganachaud F., Bernard J. Brilliant glyconanocapsules for trapping of bacteria. // Chem Commun (Camb). 2015 Aug 28;51(67):13193-13196. (IF=6.834)
Fickentscher C., Magorivska I., Janko C., Biermann M., Bilyy R., Nalli C., Tincani A., Medeghini V., Meini A., Nimmerjahn F., Schett G., Muñoz L.E., Andreoli L., Herrmann M. The Pathogenicity of Anti-b2GP1-IgG Autoantibodies Depends on Fc Glycosylation. // J Immunol Res. 2015; 2015:638129. (IF=2.934)
Bilyy R., Podhorodecki A. Сan we use rare-earth nanocrystals to target glycans for the visualization of melanoma? // Nanomedicine (Lond). 2015;10(13):1997-2000. (IF=5.413)
Zagorodko O., Bouckaert J., Dumych T., Bilyy R., Larroulet I., Yanguas Serrano A., Alvarez Dorta D., Gouin S.G., Dima S.O., Oancea F., Boukherroub R., Szunerits S. Surface Plasmon Resonance (SPR) for the Evaluation of Shear-Force-Dependent bacterial Adhesion. // Biosensors (Basel). 2015 May 26;5(2):276-287.
Yan X, Sivignon A, Yamakawa N, Crepet A, Travelet C, Borsali R, Dumych T, Li Z, Bilyy R, Deniaud D, Fleury E, Barnich N, Darfeuille-Michaud A, Gouin SG, Bouckaert J, Bernard J. Glycopolymers as Antiadhesives of E.coli Strains Inducing Inflammatory Bowel Diseases. // Biomacromolecules. 2015 Jun 8;16(6):1827-1836. (IF=5.750)
Sjöwall C, Zapf J, von Löhneysen S, Magorivska I, Biermann M, Janko C, Winkler S, Bilyy R, Schett G, Herrmann M, Muñoz LE. Altered glycosylation of complexed native IgG molecules is associated with disease activity of systemic lupus erythematosus. // Lupus. 2015 May;24(6):569-581. (IF=2.197)
Prylutska S.V. Complex of C 60 fullerene with doxorubicin as a promising agent in antitumor therapy / S.V. Prylutska, L.M. Skivka, G.V. Didenko, Y.I. Prylutskyy, M.P. Evstigneev, G.P. Potebnya, R.R. Panchuk, R.S. Stoika, U. Ritter, P. Scharff // Nanoscale Research Letters ‑ 2015 – 10, ‑ P. 499. (IF=2.584)
Boiko N.M., Klyuchivska O.Yu., Kobylinska L.I., Havrylyuk D.Ya., Mitina N.Ye., Lesyk R.B., Zaichenko O.S., Stoika R.S. Vitality and morphology of tumor cells treated with 4-thiazolidinone derivatives immobilized on nanoscale polymer carrier // Biotechnologia Acta. – 2015. – V.8, №1. – P. 39-48.
Myronovskij І. Isolation and characterization of peptides from blood serum of patients with multiple sclerosis. / Myronovskij І., Negrych N., Nehrych T., Starykovych M., Tkachenko V., Yukalo V., Storozh L., Nemchenko O, Lavryk G., Stoika R., Kit Y. // Studia Biologica. – 2015. – V. 9, №2. – P. 5–12.
Kobylinska LI, Havrylyuk DY, Ryabtseva AO, Mitina NE, Zaichenko OS, Lesyk RB, Zimenkovsky BS, Stoika RS. Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier. // Ukr Biochem J. – 2015. – V. 87, №2. – P. 122-132.
Tsivinska M.V. Isolation and properties of polyphenol oxidase from dasidiocarps of Lactarius pergamenus Fr. (Fr.) fungi / Tsivinska M. V., Antonyuk V.O., Stoika R.S. // Ukr. Biochem. J. - 2015.- V. 87, N 2. – P. 56–65.
Tsivinska M.V. Biologically active substance of methanol extracts of dried Lactarius quetus and Lactarius volemus basidiomes mushrooms: idеntification and potential functions / Tsivinska M.V., Antonyuk V.O., Panchak L.V., Klyuchivska O.Yu., Stoika R.S. // Biotechnologia Acta. (Kyiv) – 2015. – N 2, – Р. 58-68.
Годована О.І. Фармаколоґічна корекція експериментального пародонтиту на тлі системного остеопорозу / О.І. Годована, О.Ю. Ключівська, Р.С. Стойка // Acta Medica Leopoliensia. – 2015. – Т. 21, № 1. – С. 75-82.
Гренюх В., Луцик М., Стойка Р., Бабський А. Порівняльна характеристика параметрів дихання і окисного фосфорилювання у мітохондріях лімфоми NK/Ly та печінки миші. // Біологічні студії / Studia Biologica – 2015. №2. – С. 39-50.
Hodovana O.I. Klyuchivska O.Y., Stoika R.S. Level of cytokines (IL-6 and TGF-β1) in patients with generalized periodontitis at complex treatment with medications containing glycoseaminoglycanes. // Експериментальна та клінічна фізіологія і біохімія, – 2015 - Т. 21 № 4. С. 62-73
Годована О.І., Ключівська О.Ю., Годований О.В., Мітіна Н.Є., Константінова Т.Є., Заіченко О.С., Стойка Р.С. Novel polymer-mineral ZrO2-Gd2O3 radiopaque nanocomposite with hyaluronic acid coating for repair bone defects in experiment. // Acta Medica Leopoliensia. – 2015. . - Т. 21 № 3. С. 78-83.
Kоbylinska L.I. Havrylyuk D.Ya., Mitina N.E., Zаichenko A.S., Lesyk R.B., Zіmenkovsky B.S., Stoika R.S. • Biochemical indicators of hepatotoxicity in blood serum of rats under the effect of novel 4-thiazolidinone derivatives and doxorubicin and their complexes with polyethyleneglycol-containing nanoscale polymeric carrier. // Ukr. Biochem. J. 2015; Vol. 87(2); P. 122-132.
Stoika R.S. Evaluation of individual biocompatibility of osteoplastic materials used in periodontal surgery: novel approach of ex vivo biotesting of fibroblast-like cells of gums bioptate of patients (Ocena biozgodności osobniczej na materiały osteoplastyczne stosowane w chirurgii przyzębia: nowatorskie ex vivo biotestowanie fibroblastopodobnych komórek z bioptatów dziąsła pacjenta) / R. S. Stoika G. F. Biłoklicka O. I. Hodovana O. V. Godovanyi O. Yu. Klyuchivska // Stomatologia Współczesna; – 2015. – V. 22, N 4. – Р. 10–19.
Hodovana O.І. Development of novel ZrO2-Gd2O3 nanocomposite functionalized with hyaluronic acid and its application for repair of bone defects in experimental animals / O. І. Hodovana, O. Yu. Klyuchivska, O. V. Godovanyi, O. S. Miahkota, N. Ye. Mitina, T. Ye. Konstantinova, H. I. Kotsyumbas, A. S. Zaichenko, R. S. Stoika // Letters in Applied NanoBioScience, – 2015. – V. 5, Issue 3. – Р. 397-405.
Годована О.І., Ключівська О.Ю., Годований О.В., Мітіна Н.Є., Константінова Т.Є., Заіченко О.С., Стойка Р.С. Novel polymer-mineral ZrO2-Gd2O3 radiopaque nanocomposite with hyaluronic acid coating for repair bone defects in experiment. // Acta Medica Leopoliensia. - 2015. - Т. 21 № 3. С. 78-83.
Hodovana O.I. Kluchivska O.Y., Stoika R.S. Level of cytokines (IL-6 and TGF-β1) in patients with generalized periodontitis at complex treatment with medications containing glycosaminoglycanes. // Експериментальна та клінічна фізіологія і біохімія. 2015 - Т. 21 № 4. С. 62-73.
Lootsik M.D. Preparation of chitosan with high blood clotting activity and laboratory investigation of hemostatic potential of chitosan specimens. / M.D. Lootsik, R.A. Bilyy, M.M. Lutsyk, R.S. Stoika // Biotechnologia Acta. – 2015. ‑ V. 8, N 6, – P.32-40.

Монографії (розділи в монографіях):

Evaluation of ecotoxicity of waterborn metal-containing nanomaterials: novel approaches targeting metallothionein in aquatic animals / O. Stoliar, H. Falfushynska, O. Zaichenko, R. Stoika // In: Living organisms and bioanalytical approaches for detoxification and monitoring of toxic compounds. P. 177-186. Publisher: University of Rzeszow Rzeszow, Poland in cooperation with Institute of Cell Biology National Academy of Sciences of Ukraine Lviv, Ukraine. ‑ Rzeszów 2015.
Антонюк Р.В., Луцик А.Д., Антонюк В.А. Опухолевые маркеры толстого кишечника и их медицинское значение. – 20-02- 2015 Lambert Academic Publishing. ‑ 76 c.
Surface-modified magnetic nanoparticles for cell labeling / B.A. Zasonska, V. Patsula, R. Stoika, D. Horák // "The Chemistry and Physics of Engineering Materials" Editors:A. A. Berlin, R. Joswik, N.I. Vatin, / Modern Analytical Methodologies.‑ Apple Academic Press, NJ, USA. – 2015. – V. 1, Chapter 10. – P. 275-288.

Патенти на винахід (корисну модель), подані заявки:

Патент України на винахід №109177 5-[2-хлоро-3-(4-нітрофеніл)-аліліден]-4-(3,5-діарил-4,5-дигідропіразол-1-іл)-5Н-тіазол-2-они, що виявляють протипухлинну активність in vitro та in vivo та спосіб їх солюбілізації. / Гаврилюк Д.Я., Зіменковський Б.С., Лесик Р.Б., Чумак В.В., Панчук Р.Р., Стойка Р.С. Заявка a201310289, затверджено 27.07.2015, бюл. №14.
Патент на винахід №а201405945 від 30.05.2014, Міцелярна композиція на основі подвійних гідрофільних блок-кополімерів і доксорубіцину та її застосування як засобу протиракової дії. / Куницька Л.Р., Желтоножська Т.Б., Стойка Р.С., Бойко Н.М. затверджено 22.10.2015 р. бюл. №23.
Патент України на корисну модель u 2015 05278, заявка від 28.05.2015. Спосіб діагностики та визначення тяжкості перебігу розсіяного склерозу / Негрич Т.І., Кирилюк С.Я., Стойка Р.С., Кіт Ю.Я. затверджено 07.09.2015 р.
Заявка на Патент США на винахід: Bilyy R.O., Bila E.E., Kit Y.Y. Catalytic antibodies and uses thereof. WO2014037785 10

Методичні рекомендації:

Луцик М.Д. Невисокі технології отримання афінних сорбентів (Методичні розробки). – Львів, 2014. – 24 с.

2016

Articles with IF:

Prylutska S.V. Complex of C 60 fullerene with doxorubicin as a promising agent in antitumor therapy / S.V. Prylutska, L.M. Skivka, G.V. Didenko, Y.I. Prylutskyy, M.P. Evstigneev, G.P. Potebnya, R.R. Panchuk, R.S. Stoika, U. Ritter, P. Scharff // Nanoscale Research Letters ‑ 2015 – 10, ‑ P. 499 (IF=2.584)
Senkiv J. 5-Ene-4-thiazolidinones induce apoptosis in mammalian leukemia cells. J. Senkiv, N. Finiuk, D. Kaminskyy, D. Havrylyuk, M. Wojtyra, I. Kril, A. Gzella, R. Stoika, R. Lesyk // Eur J Med Chem. – 2016, ‑ Jul 19; 117 – P.33-46. (IF=3.902)
Pohrelyuk I.M. Effect of thermodiffusion nitriding on cytocompatibility of Ti-6Al-4V titanium alloy / I.M. Pohrelyuk, O.V. Tkachuk, R.V. Proskurnyak, N.M. Boiko, O.Yu. Kluchivska, R.S. Stoika // JOM (The Journal of The Minerals, Metals & Materials Society (TMS))‑ 2016, ‑ V.68, Issue 4, ‑P. 1109-1115 (IF=1.798)
Riabtseva A. Functional micelles formed by branched polymeric surfactants: Synthesis, characteristics, and application as nanoreactors and carriers / A. Riabtseva, N. Mitina, I. Grytsyna, N. Boiko, V. M. Garamus, H. Stryhanyuk, R. Stoika, A. Zaichenko // European Polymer Journal. – 2016, ‑ V. 75, ‑ P. 406-422. (IF=3.485)
Myronovkij S. Identification of Ser-Pro-Cys peptide in blood serum of multiple sclerosis patients / S. Myronovkij, N. Negrych, T. Nehrych, V. Tkachenko, S. Souchelnytskyi, R. Stoika, Yu. Kit // Protein and Peptide Letters, 2016. Vol. 23, no 9, 808-811. (IF=1.069)
Panchuk R.R. Antioxidants selenomethionine and D-pantethine decrease negative side effects of doxorubicin in NK/Ly lymphoma-bearing mice / R.R. Panchuk, N.R. Skorokhyd, Yu.S. Kozak, L.V. Lehka, V.V. Chumak, S.N. Omelyanchik, V.A. Gurinovich, A.G. Moiseenok, R.S. Stoika // Croat. Med. J. – 2016. – V. 57, N.2. – P. 180-192. (IF=1.483)
Kobylinska L.I. Putative anticancer potential of novel 4-thiazolidone derivatives: cytotoxicity toward rat glioma C6 in vitro and correlation of general toxicity with the balance of free radical oxidation in rats / L. I. Kobylinska, N. M. Boiko, R. R. Panchuk, I. I. Grytsyna, O. Yu. Klyuchivska, L. P. Biletska, R. B. Lesyk, B. Z. Zimenkovsky, R. S. Stoika // Croat. Med. J. – 2016. – V. 57, N.2. – P. 151-163. (IF=1.483)
Tomin A. Magnetic separation of apoptotic cells with lectin-conjugated microparticles (Magnetische abtrennung apoptotischer zellen mit lektin-konjugierten mikropartikeln) / A. Tomin, T. Dumych, I. Kril, V. Antonyuk, V. Chopyak, L. Munoz, R. Stoika, M. Herrmann, R. Bilyy // Materialwissenschaft und Werkstofftechnik. – 2016, ‑ V. 47, Issue 2-3 ‑ P. 189–192. (IF=0.425)
Magorivska I. Sialylation of anti-histone immunoglobulin G autoantibodies determines their capabilities to participate in the clearance of late apoptotic cells / I. Magorivska, L. E. Munoz, C. Janko, T. Dumych, J. Rech, G. Schett, F. Nimmerjahn, R. Bilyy, M. Herrmann // Clinical and Experimental Immunology, 2016, 184: 110–117. (IF=3.148)
Purwin M. Design and synthesis of novel dual-target agents for HDAC1 and CK2 inhibition / M. Purwin, J. Hernandez-Toribio, R. Panchuk, N. Skorokhyd, K. Filipiak, B. de Pascual-Teresa, A. Ramos // RSC Advances. – 2016. – V. 6, ‑ P. 66595-66608. (IF=3.840)
Maueröder C. A blast without power - cell death induced by the tuberculosis-necrotizing toxin fails to elicit adequate immune responses / C. Maueröder, R. A. Chaurio, T. Dumych, M. Podolska, M. D. Lootsik, S. Culemann, R. P. Friedrich, R. Bilyy, C. Alexiou, G. Schett, C. Berens, M. Herrmann, L. E. Munoz // Cell Death Differ. – 2016. ‑ 23(6). ‑ P. 1016-1025. (IF=8.184)
Muñoz L.E. Nanoparticles size-dependently initiate self-limiting NETosis-driven inflammation / L. E. Muñoz, R. Bilyy, M. H. Biermann, D. Kienhöfer, C. Maueröder, J. Hahn, J. M. Brauner, D. Weidner, J. Chen, M. Scharin-Mehlmann, C. Janko, R. P. Friedrich, D. Mielenz, T. Dumych, M. D. Lootsik, C. Schauer, G. Schett, M. Hoffmann, Y. Zhao, M. Herrmann // Proc Natl Acad Sci U S A. – 2016. ‑ 113(40). ‑ P. E5856-E5865. (IF=9.423)
Antonyuk R.V. Lectin purification from carp roe (Cyprinus carpio L..), research of its carbohydrate specificity and its application in histochemistry / R. V. Antonyuk, A. D. Lutsyk, V. O. Antonyuk // Romanian Journal of Morphology and Embryology, 2016, V 57. N 3 ‑ P. 985-994. (IF=0.811)

Articles in other editions included in the international science databases such as Web of Science, Scopus and others

Myronovkij S. Identification of a 48kDa form of unconventional myosin 1c in blood serum of patients with autoimmune diseases / S. Myronovkij, N. Negrych, T. Nehrych, M. J. Redowicz, S. Souchelnytskyi, R. Stoika, Yu. Kit // Biochem. Biophys. Reports. – 2016, – 5. – Р. 175-179;
Negrych N. Identification of the unique properties of IgGs and their heavy chains in blood serum of multiple sclerosis patients / N. Negrych, S. Myronovskij, T. Nehrych, R. Stoika, Yu. Kit // J. Autoimmune Disorders. – 2016. – V. 2, N 2. – Р. 1-5.
Finiuk N.S., Senkiv J.V., Riabtseva A.O., Mitina N.Y., Molochii N.I., Kitsera M.O., Avdieiev S.S., Zaichenko O.S., Stoika R.S. Modulation of temozolomide action towards rat and human glioblastoma cells in vitro by its combination with doxorubicin and immobilization with nanoscale polymeric carrier // Ukr. Biochem. J. ‑ 2016; ‑ Т. 88. ‑ P. 87-98
Kоbylinska L.I. Biochemical indicators of nephrotoxicity in blood serum of rats treated with novel 4-thiazolidinone derivatives or their complexes with polyethyleneglycol-containing nanoscale polymeric carrier / L. I. Kоbylinska, D. Ya. Havrylyuk, N. E. Mitina, A. S. Zаichenko, R. B. Lesyk, B. S. Zіmenkovsky, R. S. Stoika // Ukr. Biochem. J. – 2016. – V. 88, N 1.– P.51-60.
Lehka L.V., Panchuk R.R. , Skorokhyd N.R. , Kozak Yu.S., Rohr J., Stoika R.S. Study of inhibition of B16f10 melanoma growth in mice by landomycin A in comparison to doxorubicin // Studia Biologica. – 2016. –V. 10, N 1. – P. 5-16.
Krupak V.I., Malysheva Kh.V., Pavlenko O.К., Shafranska G.I., K. de Rooij, Löwik C.W.G.M., Stoika R.S., Korchynskyi O.G. The pttg1 is a novel inhibitor of osteogenic differentiation of mouse mesenchymal stem cells // The Animal Biology, ‑ 2016. – Vol. 18, N 1, ‑ P. 61–68.
Hodovana O.I., Martovlos A.I., Godovanyi O.V., Kluchivska O.Y, Stoika R.S. Study of the parodontoprotective effect of glycosaminoglycans by determination of biometric efficiency criteria // Dental News – 2016, N1,-P.72-77.
Hodovana O.I. Development of novel ZrO2-Gd2O3 nanocomposite functionalized with hyaluronic acid and its application for repair of bone defects in experimental animals / Hodovana O.I., Klyuchivska O.Yu., Godovanyi O.V., Miahkota O.S., Mitina N.Ye., Konstantinova T.Ye., Kotsyumbas H.I., Zaichenko O.S., Stoika R.S., // Letters In Applied NanoBioScience. – 2016. – Vol. 5, Issue 3. – P. 397-405.
Malysheva Kh.V. Generation of optimized preparations of bone morphogenetic proteins for bone regeneration / Kh.V. Malysheva, I.M. Spasyuk, O.K. Pavlenko, R.S. Stoika, O.G. Korchynskyi // Ukr. Biochem. J. ‑ 2016. ‑ V. 88, N 6. ‑ P. 59–70.
Myronovkij S. The characteristics of antibodies of mice immunized by human unconventional myosin 1C / S. Myronovkij, N.M. Boiko, V.V. Chumak, M.S. Shorobura, M.D. Lootsyk, R.S. Stoika, Yu.Ya. Kit // Ukr. Biochem. J. ‑ 2016. ‑ V. 88, N 6. ‑ P. 35-41.
Finiuk N.S., Senkiv J.V., Riabtseva A.O., Mitina N.Y., Molochii N.I., Kitsera M.O., Avdieiev S.S., Zaichenko O.S., Stoika R.S. Modulation of temozolomide action towards rat and human glioblastoma cells in vitro by its combination with doxorubicin and immobilization with nanoscale polymeric carrier // Ukr. Biochem. Journal. – 2016. ‑ V. 88, Special Issue. ‑ Р.87-98.
Lehka L.V., Panchuk R.R., Skorokhyd N.R., Kozak Yu.S., Rohr J., Stoika R.S. Study of inhibition of B16F10 melanoma growth in mice by landomycin A in comparison to doxorubicin // Studia Biologica. ‑ 2016. ‑ V. 10, N 1. ‑ P. 5-16.
O.R. Vrubel, A.R. Zin, V.O. Antonyuk. Integrated use of the bark of spindle tree (Euonymus europaea l.): investigation of lipophilic substances // Pharm journal. -2016. - N 6. ‑ P. 79-87.

2017

Articles with IF:

Mokhir A. Lysosome-targeting amplifiers of reactive oxygen species as anticancer prodrugs / A. Mokhir, S. Daum, V. Reshetnikov, M. Sisa, T. Dumych, M. D. Lootsіk, R. Bilyy, E. Bila, C. Janko, C. Alexiou, M. Herrmann, L. Sellner // Angewandte Chemie International Edition. – 2017. Vol. 6. – P. 585-… (ІF=11.994)
Englinger B. Intrinsic fluorescence of the clinically approved multikinase inhibitor nintedanib reveals lysosomal sequestration as resistance mechanism in FGFR-driven lung cancer / B. Englinger, S. Kallus, J. Senkiv, D. Heilos, L. Gabler, S. van Schoonhoven, A. Terenzi, P. Moser, Ch. Pirker, G. Timelthaler, W. Jaeger, Ch. Kowol, P. Heffeter, M. Grusch, W. Berger // Journal of Experimental and Clinical Cancer Research. – 2017. – Vо. 36. – Р.122-135. (ІF=5.182)
Panchuk R.R. Rapid generation of hydrogen peroxide contributes to the complex cell death induction by the angucycline antibiotic landomycin E / R.R. Panchuk, L.V. Lehka, A. Terenzi, B.P. Matselyukh, J. Rohr, A.K. Jha, T. Downey, I.J. Kril, I. Herbacek, S. van Schoonhoven, P. Heffeter, R.S. Stoika, W. Berger // Free Radical Biology and Medicine. – 2017. – Vol. 106. – P. 134-147 (ІF=5.606)
Finiuk N. 1.4-Thiazolidinone derivative Les-3833 effectively inhibits viability of human melanoma cells through activating apoptotic mechanisms / N. Finiuk, N. Boiko, O. Klyuchivska, L. Коbylinska, I. Kril, B. Zimenkovsky, R. Lesyk, R. Stoika // Croat Med J. – 2017. – Vol. 58(2). – P. 129-139 (ІF=1.619)
Коbylinska L.I. Differential pro-apoptotic effects of synthetic 4-thiazolidinone derivative Les-3288, doxorubicin and temozolomide in human glioma U251 cells / L.I. Коbylinska, O.Yu. Klyuchivska, I.I. Grytsyna, N.S. Finiuk, R.R. Panchuk, M.O. Starykovych, L.V. Lehka, R.B. Lesyk, B.S. Zіmenkovsky, R.S. Stoika // Croat Med J. – 2017. – Vol. 58(2). – P. 150-60 (IF=1.619)
Panchuk R.R. Tissue-protective activity of selenomethionine and D-panthetine in B16 melanoma-bearing mice under doxorubicin treatment is not connected with their ROS scavenging potential / R.R. Panchuk, N.R. Skorokhyd, Y.S. Kozak, L.V. Lehka, A.G. Moiseenok, R.S. Stoika // Croat Med J. – 2017. – Vol. 58(2). – P. 171-84 (IF=1.619)
Horák D. PEGylation controls attachment and engulfment of monodisperse magnetic poly(2-hydroxyethyl methacrylate) microspheres by murine J774.2 macrophages / D. Horák, H. Hlídková, O. Klyuchivska, I. Grytsyna, R. Stoika // Applied Surface Science. – 2017. - Vol. 426. - P. 315-324 (IF=3.387)
Horak D. Magnetic poly(2-hydroxyethyl methacrylate) microspheres for affinity purification of antibodies for early diagnosis of multiples clerosis patients / D. Horak, H. Hlidkova, Yu. Kit, R. Stoika, V. Antonyuk, S. Myronovsky // Biosci. Rep. – 2017. – Vol. 37(2). - Р. 1–10 (IF=2.906)
Kucinska-Lipka J. The influence of calcium glycerophosphate (GPCa) modifier on physicochemical, mechanical, and biological performance of polyurethanes applicable as biomaterials for bone tissue scaffolds fabrication. / J. Kucinska-Lipka, I. Gubanska, O. Korchynskyi, K. Malysheva, M. Kostrzewa, D. Włodarczyk, J. Karczewski, H. Janik // Polymers. – 2017. – Vol. 9(8). P. 329. (IF=3.364)

Articles in other editions included in the international science databases such as Web of Science, Scopus and others:

Malysheva Kh.V. 4-thiazolidinone–based derivatives rescue TNFα-inhibited osteoblast differentiation in mouse mesenchymal precursor cells / Kh.V. Malysheva, N.S. Finiuk, O.K. Pavlenko, D.Ya. Havrylyuk, R.B. Lesyk, R.S. Stoika, O.G. Korchynskyi // Ukr Biochem J. – 2017 ‑ Vol. 89 (Special Issue). – P. 112–123.
N.S. Finiuk Antineoplastic activity of novel thiazole derivatives / N.S. Finiuk, V.P. Hreniuh, Yu.V. Ostapiuk, V.S. Matiychuk, D.A. Frolov, M.D. Obushak, R.S. Stoika, A.M. Babsky // Biopolym. Cell. – 2017. ‑ Vol. 33(2). – P. 135-146
Kit Yu. Anti-histone H1 IgGs possess proliferative activity towards humanT-leukaemia CEM cells / Yu. Kit, I. Magorivska, R. Bilyi, S. Myronovskij, R. Stoika // Exp. Oncol. ‑ 2017. ‑ Vol. 39(1). – P. 36–41
Kit Yu. Anti-DNA (“Cationic”) and Ani-Histone (“Anionic”) Auto-Antibodies: Two Sides of One Coin? / Kit Yu. // J. Translational Proteome Res. – 2017. ‑ V. 3. – P. 1-5
Zelisko N.I. Screening of spiro-substituted thiopyrano[2,3-d]thiazoles for their cytotoxic action on tumor cells / N.I. Zelisko, N.S. Finiuk, V.M. Shvets, Yu.O. Medvid , R.S. Stoika, R.B. Lesyk // Biopolym. Cell. ‑ 2017. ‑ Vol. 33. (N 4). P. 282–290
Myronovskij S. Characteristics of Potential Protein Biomarkers Extracted with 10% TCA from Blood Serum of Non-Hodgkin’s Lymphoma and Multiple Myeloma Patients / S. Myronovskij, O. Shalay, V. Spivak, R. Stoika, Yu. Kit // Int J Mol Cell Med.‑ 2017. ‑ Vol 6(4). – Р. 1-4

Articles in professional publications of Ukraine:

Malysheva Kh. shRNA-mediated knockdown of pituitary tumor transforming gene 1 (PTTG1): optimization of the effect and targeting of early osteoblast differentiation in murine mesenchymal stem cells / Kh. Malysheva, O. Pavlenko, R. Stoika, O. Korchynskyi // Animal Biology. – 2017. ‑ Vol. 19(2). – P. 70-8
N.O. Negrych, S.L. Myronovskij, T.I. Nehrych, Yu.Ya. Kit, R.S. Stoika. Modern aspects of B lymphocytes in the pathogenesis of multiple sclerosis: the use of B-cell biomarkers in clinical practice // International neurological journal – 2017. - Vol. 3(89). – P. 38-44
Stoika R. How The Multifunctional Nanocarrier Makes The Medicine «Smart»? // Proceedings of the Shevchenko Scientific Society Medicine. – 2017. ‑ V. 49 (1). – P. 41, 48-52

Monographs:

Multifunctional nanomaterials for biology and medicine: molecular design, synthesis and application”.(Ed. R.S. Stoika) - 2017. – 364 p.

Methodical recomendations:

Antonyuk V.O., Panchak L.V. Methodical recommendations on pharmacognosy for pharmacists-interns in the speciality “General Pharmacy”. – Lviv. – 2017. – 44 p.

Ukrainian | English

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Regulation of Cell Proliferation and Apoptosis

Research Grants

International Grants

"The role of transforming growth factors in the mechanisms of hyperthermia action on the proliferation of tumor and normal mammalian cells" (CDA-NIS Program, National Cancer Institute (NIH, USA), 1995-1998) – Principal Investigator – Stoika R.S.
"Expression of Smad tumor suppressor genes in breast and colorectal cancers" (The Royal Swedish Academy of Sciences (Sweden), 1997-1998) – Co-Principal Investigator – Stoika R.S.
"Transforming growth factor beta signalling in human breast cancer: study of ligand, receptors, Smad proteins expression and intactness of the signalling" (The Royal Swedish Academy of Sciences (Sweden), 2000-2001) – Co-Principal Investigator – Stoika R.S.
"Changes in plasma membrane glycoproteins at cell death and their role in development of autoimmune diseases" (Grant from Ministry of education and science of Federative Republic of Germany for collaboration with the Ministry of education and science of Ukraine 2009-2011) – Co-Principal Investigator – Stoika R.S.
"Functionally active colloid carriers for chemical, biological and microelectronic technologies" (Grant #1930 from Science and Technology Center of Ukraine, 2004-2006) – Principal Investigator – Dr.Chem.Sci. Zaichenko O.S., team leader – Stoika R.S., corresponding member of NAS of Ukraine.
"Information videoscope of ultrahigh resolution for investigation of programmed death (apoptosis) of human tumor cells" (Grant #1702 from Science and Technology Center of Ukraine, 2004-2006) – Co-Principal Investigator – Stoika R.S.
"Novel functiоnal luminescent polymer and hybrid nanoparticles and nano-assembles with directed chemical and physical characteristics" (Grant #4140 from Science and Technology Center of Ukraine, 2007-2008) – Principal Investigator – Dr.Chem.Sci. Zaichenko O.S., team leader – Stoika R.S., corresponding member of NAS of Ukraine.
"Nаnоscale systems fоr delivery of anticancer substances based on novel luminescent and X-ray detectable oligoelectrolytes" (Grant #4953 from Science and Technology Center of Ukraine, 2009-2011) – Principal Investigator – Stoika R.S., corresponding member of NAS of Ukraine, team leader – assoc. prof. Zaichenko O.S.
"Novel functional nanomaterials for enhancement of the efficiency of chemotherapy" (Grant #6256 from Science and Technology Center of Ukraine, 2017-2019) – Co-Principal Investigator – Stoika R.S.
"Lаndomycin А – a new preparation for chemotherapy of drug-resistant tumors: production, preclinical study, marketing" (CRDF grаnt №FSCX-14-60609-0 (USА – Ministry of education and science of Ukraine), 2015-2017) – Principal Investigator – Stoika R.S.
Grant for 100 best scientific teams that collaborate with Shandong Academy of Sciences (Іnstitute of Biology, Jinan, Shandong province, Peoples Republic of China, 2020-2022) – Principal Investigator – Stoika R.S., corr. member of NAS of Ukraine.
Bilateral Ukraine-Austria cooperation project "New approaches to the treatment of metastatic tumors resistant to chemotherapy, based on the angucycline antibiotic landomycin A immobilized on a poly(2)-oxazoline carrier" – State registration number 0119U102525, 2019-2020 – Scientific supervisor – Panchuk R.R., Dr.Sc. Co-executor in Austria – Institute for Cancer Research, Medical University of Vienna.
Bilateral Ukraine-Austria cooperation project “Dual action on specific vulnerabilities of malignant cells by new thiosemicarbazone derivatives” – Contract dated 12.09.2023 No.M/88-2023, Contract dated 22.05.2024 No.M/30-2024, 12.09.2023-31.12.2024 – Scientific supervisor – Stoika R.S. Corresponding Member of the NAS of Ukraine, Doctor of Biological Sciences, Professor, Head of Department. – Co-executor in Austria – Institute of Cancer Research, Medical University of Vienna.

Grants from Ukrainian Organizations

"Study of signaling mechanisms of apoptosis induction depending on the types of inducer and target cells in vitro and in vivo" – NASU project (2006-2010) – scientific supervisor (PI) – corresponding member of NASU R.S. Stoyka
"Mechanisms of action in vitro and in vivo of new compounds with proapoptotic activity, ways to increase the effectiveness of their antineoplastic action and justification of new approaches in diagnostics and assessment of the development of autoimmune and hemato-oncological diseases" – NASU project No.: iii-3-12 (2011-2016) – scientific supervisor – corresponding member of NASU R.S. Stoyka
"Increasing the efficiency of traditional and experimental drugs in vitro and in vivo by immobilizing them on nano-sized carriers and combining them with antioxidants" – NASU Project No.: III-3-17 (2017-2021) – scientific supervisor – corresponding member of NASU Stoika R.S.
"Increasing the efficiency of target gene delivery to plants using new nano-sized polycationic polymers" – jointly with the state institution "Institute of Food Biotechnology and Genomics" NASU) project No.32f, 2015-2019 – scientific supervisor – corresponding member of NASU Stoika R.S.
"New approaches to overcome tumor resistance to chemotherapy caused by deregulation of the cellular glutathione system" – Ukrainian-Belarusian project of the DFFD of Ukraine (2019-2020) – supervisor – corresponding member of the NAS of Ukraine Stoika R.S.
"Antitumor effect of chemotherapeutic drugs under the conditions of their delivery by new nanocomposite carriers functionalized with N-acylethanolamines" – Project No.5.9.1.50 of the state target scientific and technical program "nanotechnologies and nanomaterials" (2010-2014) – PI and responsible executor – Ph.D. Panchuk R.R.
"New biocompatible polymer nanocomposites with encapsulated fluorescein: their biofunctionalization and effect on cells" – Project No.101/10N of the Targeted Comprehensive Program of Fundamental Research of the National Academy of Sciences of Ukraine "Nanostructural Systems, Nanomaterials, Nanotechnologies" (2010-2014) – PI and Responsible Executor – Ph.D. Panchuk R.R.
"Study of Molecular Mechanisms of Action and Antitumor Activity of Novel Biologically Active Substances" – Presidential Grant for Support of Research of Young Scientists No.gp/f32/192-2011 (2011) – Responsible Executor – Ph.D. Panchuk R.R.
"New Nanoscale Drug Delivery Systems to Target Cells" – Presidential Grant for Support of Research of Young Scientists No.Gp/F36/408-2012 (2012) – PI and Responsible Executor – Ph.D. Panchuk R.R.
"Mechanisms of cytotoxic activity of angucycline antibiotic landomycin E on tumor cells" – Ukrainian-Austrian bilateral projects – No.m/577-2011 and m/246-2012 (2011-2012) – PI and responsible executor – Ph.D. Panchuk R.R.
"Inhibition of growth of experimental tumors with high aerobic glycolysis: development of new approaches based on metabolomics" – Presidential Grant for Gifted Youth (2013) – PI and responsible executor – Ph.D. Panchuk R.R.
"Use of redox-modulating compounds to increase the selectivity of action of new antitumor drugs" – Ukrainian-Belarusian bilateral project No.F54/185-2013 (2013) – responsible executor – Ph.D. Panchuk R.R.
"Enhancement of the antineoplastic activity of novel antitumor drugs using combined chemotherapy approaches and improved drug delivery to malignant cells" – Ukrainian-Austrian bilateral project No.M/3-2013 and No.M/112-2014 (2013-2014) – responsible executor – Ph.D. Panchuk R.R.
"Combining metabolomics approaches and new chemotherapeutic agents in the treatment of lymphomas and leukemias" – Presidential Grant to Support Research of Young Scientists No.F61/96-2015 (2015) - responsible executor – Ph.D. Panchuk R.R.
"Redox modulation of the glutathione system in tumor cells with different sensitivity to chemotherapy". Ukrainian-Belarusian bilateral project No.f73/85-2016 and f73/55-2017 (2016-2017) – responsible executor – Ph.D. Panchuk R.R.
"Molecular mechanisms of the modulatory action of antioxidants on the therapeutic activity of new anticancer drugs" – Presidential Grant to Support Research of Young Scientists No.F70/79-2017 (2017) – responsible executor – Ph.D. Panchuk R.R.
"New approaches to the treatment of metastatic tumors resistant to chemotherapy based on the angucycline antibiotic landomycin A immobilized on a poly(2)-oxazoline carrier" – (2019-2020) Ukrainian-Austrian project – supervisor – Dr.Sc. Panchuk R.R.
"Enhancement of the antitumor effect of landomycin A by immobilization on a poly-2-oxazoline nanoplatform: in vitro and in vivo effects" – Grant of the NAS of Ukraine to support research laboratories of young scientists (2018-2019) – supervisor – Dr.Sc. Panchuk R.R.
"New biochemical approaches for assessing the ecotoxicity of metal-containing nanomaterials based on determining the characteristics of metallothioneins of aquatic animal species" - National Academy of Sciences of Ukraine (2010-2014) – Project leader – Corresponding Member of NAS U Stoika R.S., Co-leader – Doctor of Biological Sciences Stolyar O.B., Group leader – Doctor of Chemical Sciences Zaichenko O.S.
"Use of effective oligoelectrolyte systems for gene delivery for the purpose of genetic transformation of plant cells" – National Academy of Sciences of Ukraine (2010-2014). Project leader – Corresponding Member of NAS U Stoika R.S., Group leader – Doctor of Chemical Sciences Zaichenko O.S.
"Creation of unified systems based on functional nanocarriers for the detection and isolation of pathogenic factors of biological origin" – National Academy of Sciences of Ukraine (2010-2014) – Co-leader of the project – Corresponding Member of the NAS of Ukraine Stoika R.S.
"New biocompatible nanocomposites with encapsulated fluorescein: their biofunctionalization and effect on cells" – National Academy of Sciences of Ukraine (2010-2014) – Project leader – Corresponding Member of the NAS of Ukraine Stoika R.S., Group leader – Doctor of Chemical Sciences Zaichenko O.S.
"Antitumor effect of chemotherapeutic drugs when delivered by new nanocomposite carriers functionalized with N-acylethaneamines" – National Academy of Sciences of Ukraine (2010-2014) – Co-leader of the project – Corresponding Member of the NAS of Ukraine Stoika R.S., group leader – Doctor of Chemical Sciences Zaichenko O.S., executor – Doctor of Biological Sciences Panchuk R.R.
"Mechanisms of action in vitro and in vivo of new compounds with proapoptotic activity, ways to increase the effectiveness of their antineoplastic action and justification of new approaches in the diagnosis and assessment of the development of autoimmune and hemato-oncological diseases" – NAS of Ukraine, state registration number 0112U002174 (2012-2016) – supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
"Improving the efficiency of target gene delivery to plants using new nanosized polycationic polymers" - NAS of Ukraine, grant of the targeted complex interdisciplinary scientific research program of NAS of Ukraine "Molecular and cellular biotechnologies for the needs of medicine, industry and agriculture" – 2017-2019 supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
"Target gene delivery into mammalian cells using novel native biocompatible polymeric nanomaterials" – Program-targeted and competitive topics of the NAS of Ukraine - Grants of the NAS of Ukraine to research laboratories/groups of young scientists of the NAS of Ukraine for conducting research in priority areas of science and technology development 2020-2021, state registration number 0120U100197 (2020-2021) – supervisor - Finiuk N.S., Ph.D.
"Interrelations between immunomodulatory and antitumor actions of new heterocyclic compounds (thiazole and 4-thiazolidinone derivatives)". Program-targeted and competitive topics of the NAS of Ukraine, state registration number 0120U103077 (2020-2022) – supervisor – Dr.Sc. Lootsyk M.D.
"Novel Dual Inhibitors of Casein Kinase 2 and Histone Deacetylase for Targeted Tumor Chemotherapy" - NAS of Ukraine, grant to research groups of young scientists of NAS of Ukraine for conducting research in priority areas of development of science and technology 2021-2022, state registration number 0121U110732 (2021-2022) – Scientific supervisor – Dr.Sc. Panchuk R.R.
Departmental topic "The role of immunomodulatory in the antitumor action of traditional and experimental medicines at their delivery by polymeric nanocarriers to various target cells in vitro and in vivo" – state budget topic of the NAS of Ukraine, state registration number 0122U002240 (2022-2026) – supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
"Enhancement of biological activity of berberine-like derivatives via their covalent and non-covalent modifications" - NAS of Ukraine, under the budget program "Support for the development of priority areas of scientific research" – OK 0222U005151 (2022) – supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
"Anti-inflammatory and wound-healing agent containing the composition of the cannabimimetic N-stearoylethanolamine with chitosan hydrogel" – Grant of the National Research Foundation of Ukraine, competition "Science for the reconstruction of Ukraine in the war and post-war periods", registration number 2022.01/0207, state registration number 0123U103497 (2023-2024) – supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
"Molecular design, synthesis and preclinical testing of new thiazole and thiazolidine derivatives with selective action on cells of myeloproliferative neoplasms" – Grant of the National Research Foundation of Ukraine, competition "Advanced Science in Ukraine", registration number 2023.03/0104, state registration number 0124U003835 (2024-2026) – supervisor (PI) – Finiuk N.S., Ph.D.

Ukrainian | English

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Regulation of Cell Proliferation and Apoptosis

Personnel:

Rostyslav Stoika – Head of the Department, D. Sci. (Biol.), Professor, Corresponding Member of NAS of Ukraine;
Maxim Lutsik – Senior Scientist, Dr.Sci. (Biol.), Professor;
Rostysav Panchuk – Senior Researcher, Dr.Sci. (Biol.);
Volodymyr Antonyuk – Senior Researcher, Dr.Sci. (Pharm.), Professor;
Nataliya Finiuk – Researcher, PhD;
Julia Senkiv – Researcher, PhD;
Nataliya Kashchak – Junior Research Scientist, PhD;
Yuliia Kozak – Junior Research Scientist, PhD;
Nazar Manko – Junior Research Scientist, PhD;
Iryna Ivasechko – Junior Research Scientist, PhD;
Nadiya Skorohid – Lead Engineer, PhD;
Olha Klyuchivska – Lead Engineer;
Mykola Klishch – Postgraduate student.

Laboratory

Laboratory of the Mechanisms of Intercellular Interaction, Head – Nataliya Finiuk, PhD

Ukrainian | English

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Cell Signaling

Head of Department – Oleh STASYK, PhD, D.Sc., Senior Researcher.

Department of Cell Signaling (the name was approved by the Academic Council of the Institute of Biochemistry of the NAS of Ukraine on October 19th, 2000) is the successor of the Department of Biochemistry of Cell Differentiation, established in 1969 as part of the O.V. Palladin Institute of Biochemistry of the AS of Ukraine, since 1974 as a part of the Lviv Department of the O.V. Palladin Institute of Biochemistry of the AS of Ukraine, in 1992 renamed as the Department of Cell Regulatory Systems of the O.V. Palladin Institute of Biochemistry of the NAS of Ukraine. From the moment of its establishment until 1998, the department was headed by the Honored Worker of Science and Technology of Ukraine, Professor Stepan Kusen, and from 1999 to 2006, the head of the department (since 2000 as part of the newly established Institute of Cell Biology of the NAS of Ukraine) was Dr. Lyudmyla Drobot.

Department of Cell Signaling (DCS) employs: 1 leading researcher (0.5 part-time position), 3 junior researchers, 1 graduate student and 1 engineer. Since 2007 the employees have defended 5 PhD theses (Vynnytska-Myronovska B., Kurlishchuk Y., Pavlyk Y., Karatsai O., Chen O.) and 1 D.Sc. dissertation (Stasyk O.).

DCS conducts international-level research on the development of new approaches to metabolic therapy of malignant tumors based on amino acid starvation. A number of new combined approaches have been proposed, which should make the corresponding therapy more effective and selective. In particular, it was established for the first time in studies conducted during 2007-2025 on a wide range of cell models that:

the level of endogenous enzymes of arginine amino acid biosynthesis in tumor cells of different types does not determine their sensitivity to arginine starvation in vitro in monolayer culture or 3D spheroid culture;
the sensitivity of malignant cells to starvation for individual amino acids is significantly reduced in 3D cultures compared to 2D cultures;
arginine deficiency selectively and significantly inhibits the motility, invasive and clonogenic potential of different types of tumors;
inhibitors of autophagy, proteasomal degradation, inducers of oxidative stress, and inducers of endoplasmic reticulum stress enhance the effect of monotherapy based on arginine deficiency and radiosensitize a number of tumor types;
the plant-derived arginine analogues, canavanine and indospicine, significantly and selectively enhance the proapoptotic potential of metabolic therapy based on arginine deprivation, causing acute endoplasmic reticulum stress and deregulation of a number of cell signaling mechanisms, in particular, the mTOR and MAPK pathways;
indospicine, which accumulates in the biomass of the subtropical legume plant Indigofera spicata, is not subject to degradation by recombinant enzymes-hydrolases human arginase I and bacterial arginine deiminase, which are used in metabolic therapy in clinics.

Testing of combination therapy approaches developed on in vitro cultured cell models in laboratory mouse models with transplanted tumors is in progress.

Since 2021, the Department has been actively and successfully developing an applied studies on obtaining monoclonal antibodies to protein-antigens of biotechnological and medical importance using hybridoma technology, which resulted in the implementation of the antibodies obtained in the DCS as part of the rapid lateral flow tests of the company "ХEMA" for the diagnosis of coronavirus (2021) and tuberculosis (2024) pathogens by immunochromatographic analysis, as evidenced by the corresponding acts of implementation.

DCS has created a new model of neurodegenerative Parkinson's disease based on recombinant strains of the yeast Ogataea polymorpha, expressing the cytotoxic human alpha-synuclein protein, which is involved in the pathogenesis of this disease. Research has begun on studying the effects of autophagy modulation, exogenous polyamines, metal ions, oxidative stress and other factors on the cytotoxicity of the corresponding protein.

In recent years, the Department has initiated research aimed at identifying new informative markers related to the diagnosis, prognosis and monitoring of patients with traumatic brain injury (TBI). A bank of blood plasma samples from patients with TBI is being created. The aim of this work is to create prototypes of rapid tests for TBI that can be used in frontline regions with disrupted medical infrastructure, by military medics and in sports medicine.

The Department is equipped with boxes for working with microorganisms and human and animal in vitro cell cultures. DCS also has its own cryobank of pseudonormal and malignantly transformed human and animal cell lines and a collection of model strains of microorganisms (bacteria, yeasts). DCS has its own modernized animal facility, which houses C57Bl/6 and BALB/c mice, allowing studies of the toxicity of various compounds, the effectiveness of antitumor therapy in vivo, and the production of monoclonal antibodies in animals. In recent years, the Department has undergone a significant upgrade to its equipment. From several sources, in particular, due to the funding of the project No. 2023.04/0048 of the National Research Fund of Ukraine, biomedical boxes for sterile work, an automatic hematological analyzer, an inverted microscope, a microplate photometer, laboratory centrifuges, a PCR machine, etc., have been purchased and actively exploited.

Ukrainian | English

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Cell Signaling

The main scientific interests of the Department are:

Search for new approaches to metabolic anticancer therapy, in particular, based on artificial starvation for individual amino acids and combined approaches;

Research on natural analogues of amino acids as potential antitumor and antimicrobial agents;

Development of new model of Parkinson's disease based on methylotrophic yeasts;

Designing stable hybridoma clones and monoclonal antibodies against protein-antigens of biotechnological and medical importance to develop new diagnostic systems.

Ukrainian | English

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Cell Signaling

Publications

Year	Published scientific articles		Total impact factor
Year	All	Including articles in the international journals	Total impact factor
2008	3		___	View publications
2009	5		___	View publications
2010	3		___	View publications
2011	11		12.795	View publications
2012	5		13.262	View publications
2013	4		7.464	View publications
2014	5		6.876	View publications
2015	4		8.659	View publications
2016	8	3	12.300	View publications
2017	4	3	13.940	View publications
2018	5	5	17.590	View publications
2019	2	1	10.659	View publications
2020	6	5	22.000	View publications
2021	5	4	30.700	View publications
2022	1		___	View publication
2023	___		___
2024	4	1	4.500	View publication
2025	3	2	8.800	View publication

2008

Stasyk O.G., Maidan M., Stasyk O.V., Van Dijck P., Thevelein J.M., Sibirny A.A. Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha // Eukaryotic Cell. -2008.- V.7, № 4. – P.735-746.
Ishchuk O.P., Voronovsky A.Y., Stasyk O.V., Gayda G.Z., Gonchar M.V., Abbas C.A., Sibirny A.A. Improvement of xylose high-temperature fermentation in Hansenula polymorpha due to overexpression of the PDC1 gene coding for pyruvate decarboxylase // FEMS Yeast Research. – 2008. – V.8, № 7. – P.1164-1174.
Sybirna N., Brodiak I., Ferents I., Barska M. The effect of L-arginine on the oxidative modification of plasma fibrinogen under experimental diabetes mellitus in rats // Annal. Univers. Mariae Curie-Sklodovska, sectio AA Biologia – 2008. – V. IX,– P. 115-118.

2009

Havrylov S., Rzhepetskyy Yu., Malinowska A., Drobot L., Rendowicz M.J. Proteins recruited by SH3 domains of Ruk/CIN85 adaptor identified by LC-MS/MS // Proteome Science.-2009.-Vol. 7, N21.-P. 1-19.
O.I. Basaraba, M.L. Barska, Ya.P. Bobak, H.Yu. Shuvayeva, S.M. Marchenko, V. R. Savran, N.A. Volodko, V.L. Buchman, L.B. Drobot. Expression of (RUK/CIN85)/(CD2AP/CMS) family adapter proteins in human breast adenocarcinoma // Clin. and Experim. Potol.-2009- V. 9, № 4. – P. 125-130.
Rzhepetskyy Y.А., Svinchuk V.V., Buchman V.L.,Drobot L.B. Comparative analysis of Ruk/CIN85 phosphorylation in rat pheochromocytome cell line PC12 under growth factors stimulation// Clinical and eksperimental pathology. 2009.- V. VIII, №2.- p. 64-67.
Brodyak I.V., Zdioruk N.І., Barska M.L., Vovk O.І., Sybirna N.O. Lectincytochemical analysis of mononuclear leukocyte plasmatic membrane sialocontaining glycoprotein of peripheral blood under type 1 diabetes mellitus // Med.Chim. – 2009. – № 4. – P. 76–79 (Ukrainian).
Zdioruk M.I., Barska M.L., Brodyak I.V., Vovk O.I. Urbanovych A.M., Sybirna N.O. Influence of wortmannin on aggregation ability of neutrophylic granulocytes under type 1 diabetes mellitus / Studia Biologica.– 2009. –V.3, №2. – P. 133–140 (Ukrainian).

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