Institute of Cell Biology National Academy of Sciences of Ukraine

 

Department of Regulation of Cell Proliferation and Apoptosis

 

Research Grants

 

International Grants

1. "The role of transforming growth factors in the mechanisms of hyperthermia action on the proliferation of tumor and normal mammalian cells" (CDA-NIS Program, National Cancer Institute (NIH, USA), 1995-1998) – Principal Investigator – Stoika R.S.
2. "Expression of Smad tumor suppressor genes in breast and colorectal cancers" (The Royal Swedish Academy of Sciences (Sweden), 1997-1998) – Co-Principal Investigator – Stoika R.S.
3. "Transforming growth factor beta signalling in human breast cancer: study of ligand, receptors, Smad proteins expression and intactness of the signalling" (The Royal Swedish Academy of Sciences (Sweden), 2000-2001) – Co-Principal Investigator – Stoika R.S.
4. "Changes in plasma membrane glycoproteins at cell death and their role in development of autoimmune diseases" (Grant from Ministry of education and science of Federative Republic of Germany for collaboration with the Ministry of education and science of Ukraine 2009-2011) – Co-Principal Investigator – Stoika R.S.
5. "Functionally active colloid carriers for chemical, biological and microelectronic technologies" (Grant #1930 from Science and Technology Center of Ukraine, 2004-2006) – Principal Investigator – Dr.Chem.Sci. Zaichenko O.S., team leader – Stoika R.S., corresponding member of NAS of Ukraine.
6. "Information videoscope of ultrahigh resolution for investigation of programmed death (apoptosis) of human tumor cells" (Grant #1702 from Science and Technology Center of Ukraine, 2004-2006) – Co-Principal Investigator – Stoika R.S.
7. "Novel functiоnal luminescent polymer and hybrid nanoparticles and nano-assembles with directed chemical and physical characteristics" (Grant #4140 from Science and Technology Center of Ukraine, 2007-2008) – Principal Investigator – Dr.Chem.Sci. Zaichenko O.S., team leader – Stoika R.S., corresponding member of NAS of Ukraine.
8. "Nаnоscale systems fоr delivery of anticancer substances based on novel luminescent and X-ray detectable oligoelectrolytes" (Grant #4953 from Science and Technology Center of Ukraine, 2009-2011) – Principal Investigator – Stoika R.S., corresponding member of NAS of Ukraine, team leader – assoc. prof. Zaichenko O.S.
9. "Novel functional nanomaterials for enhancement of the efficiency of chemotherapy" (Grant #6256 from Science and Technology Center of Ukraine, 2017-2019) – Co-Principal Investigator – Stoika R.S.
10. "Lаndomycin А – a new preparation for chemotherapy of drug-resistant tumors: production, preclinical study, marketing" (CRDF grаnt №FSCX-14-60609-0 (USА – Ministry of education and science of Ukraine), 2015-2017) – Principal Investigator – Stoika R.S.
11. Grant for 100 best scientific teams that collaborate with Shandong Academy of Sciences (Іnstitute of Biology, Jinan, Shandong province, Peoples Republic of China, 2020-2022) – Principal Investigator – Stoika R.S., corr. member of NAS of Ukraine.
12. Bilateral Ukraine-Austria cooperation project "New approaches to the treatment of metastatic tumors resistant to chemotherapy, based on the angucycline antibiotic landomycin A immobilized on a poly(2)-oxazoline carrier" – State registration number 0119U102525, 2019-2020 – Scientific supervisor – Panchuk R.R., Dr.Sc. Co-executor in Austria – Institute for Cancer Research, Medical University of Vienna.
13. Bilateral Ukraine-Austria cooperation project “Dual action on specific vulnerabilities of malignant cells by new thiosemicarbazone derivatives” – Contract dated 12.09.2023 No.M/88-2023, Contract dated 22.05.2024 No.M/30-2024, 12.09.2023-31.12.2024 – Scientific supervisor – Stoika R.S. Corresponding Member of the NAS of Ukraine, Doctor of Biological Sciences, Professor, Head of Department. – Co-executor in Austria – Institute of Cancer Research, Medical University of Vienna.

 

Grants from Ukrainian Organizations

1. "Study of signaling mechanisms of apoptosis induction depending on the types of inducer and target cells in vitro and in vivo" – NASU project (2006-2010) – scientific supervisor (PI) – corresponding member of NASU R.S. Stoyka
2. "Mechanisms of action in vitro and in vivo of new compounds with proapoptotic activity, ways to increase the effectiveness of their antineoplastic action and justification of new approaches in diagnostics and assessment of the development of autoimmune and hemato-oncological diseases" – NASU project No.: iii-3-12 (2011-2016) – scientific supervisor – corresponding member of NASU R.S. Stoyka
3. "Increasing the efficiency of traditional and experimental drugs in vitro and in vivo by immobilizing them on nano-sized carriers and combining them with antioxidants" – NASU Project No.: III-3-17 (2017-2021) – scientific supervisor – corresponding member of NASU Stoika R.S.
4. "Increasing the efficiency of target gene delivery to plants using new nano-sized polycationic polymers" – jointly with the state institution "Institute of Food Biotechnology and Genomics" NASU) project No.32f, 2015-2019 – scientific supervisor – corresponding member of NASU Stoika R.S.
5. "New approaches to overcome tumor resistance to chemotherapy caused by deregulation of the cellular glutathione system" – Ukrainian-Belarusian project of the DFFD of Ukraine (2019-2020) – supervisor – corresponding member of the NAS of Ukraine Stoika R.S.
6. "Antitumor effect of chemotherapeutic drugs under the conditions of their delivery by new nanocomposite carriers functionalized with N-acylethanolamines" – Project No.5.9.1.50 of the state target scientific and technical program "nanotechnologies and nanomaterials" (2010-2014) – PI and responsible executor – Ph.D. Panchuk R.R.
7. "New biocompatible polymer nanocomposites with encapsulated fluorescein: their biofunctionalization and effect on cells" – Project No.101/10N of the Targeted Comprehensive Program of Fundamental Research of the National Academy of Sciences of Ukraine "Nanostructural Systems, Nanomaterials, Nanotechnologies" (2010-2014) – PI and Responsible Executor – Ph.D. Panchuk R.R.
8. "Study of Molecular Mechanisms of Action and Antitumor Activity of Novel Biologically Active Substances" – Presidential Grant for Support of Research of Young Scientists No.gp/f32/192-2011 (2011) – Responsible Executor – Ph.D. Panchuk R.R.
9. "New Nanoscale Drug Delivery Systems to Target Cells" – Presidential Grant for Support of Research of Young Scientists No.Gp/F36/408-2012 (2012) – PI and Responsible Executor – Ph.D. Panchuk R.R.
10. "Mechanisms of cytotoxic activity of angucycline antibiotic landomycin E on tumor cells" – Ukrainian-Austrian bilateral projects – No.m/577-2011 and m/246-2012 (2011-2012) – PI and responsible executor – Ph.D. Panchuk R.R.
11. "Inhibition of growth of experimental tumors with high aerobic glycolysis: development of new approaches based on metabolomics" – Presidential Grant for Gifted Youth (2013) – PI and responsible executor – Ph.D. Panchuk R.R.
12. "Use of redox-modulating compounds to increase the selectivity of action of new antitumor drugs" – Ukrainian-Belarusian bilateral project No.F54/185-2013 (2013) – responsible executor – Ph.D. Panchuk R.R.
13. "Enhancement of the antineoplastic activity of novel antitumor drugs using combined chemotherapy approaches and improved drug delivery to malignant cells" – Ukrainian-Austrian bilateral project No.M/3-2013 and No.M/112-2014 (2013-2014) – responsible executor – Ph.D. Panchuk R.R.
14. "Combining metabolomics approaches and new chemotherapeutic agents in the treatment of lymphomas and leukemias" – Presidential Grant to Support Research of Young Scientists No.F61/96-2015 (2015) - responsible executor – Ph.D. Panchuk R.R.
15. "Redox modulation of the glutathione system in tumor cells with different sensitivity to chemotherapy". Ukrainian-Belarusian bilateral project No.f73/85-2016 and f73/55-2017 (2016-2017) – responsible executor – Ph.D. Panchuk R.R.
16. "Molecular mechanisms of the modulatory action of antioxidants on the therapeutic activity of new anticancer drugs" – Presidential Grant to Support Research of Young Scientists No.F70/79-2017 (2017) – responsible executor – Ph.D. Panchuk R.R.
17. "New approaches to the treatment of metastatic tumors resistant to chemotherapy based on the angucycline antibiotic landomycin A immobilized on a poly(2)-oxazoline carrier" – (2019-2020) Ukrainian-Austrian project – supervisor – Dr.Sc. Panchuk R.R.
18. "Enhancement of the antitumor effect of landomycin A by immobilization on a poly-2-oxazoline nanoplatform: in vitro and in vivo effects" – Grant of the NAS of Ukraine to support research laboratories of young scientists (2018-2019) – supervisor – Dr.Sc. Panchuk R.R.
19. "New biochemical approaches for assessing the ecotoxicity of metal-containing nanomaterials based on determining the characteristics of metallothioneins of aquatic animal species" - National Academy of Sciences of Ukraine (2010-2014) – Project leader – Corresponding Member of NAS U Stoika R.S., Co-leader – Doctor of Biological Sciences Stolyar O.B., Group leader – Doctor of Chemical Sciences Zaichenko O.S.
20. "Use of effective oligoelectrolyte systems for gene delivery for the purpose of genetic transformation of plant cells" – National Academy of Sciences of Ukraine (2010-2014). Project leader – Corresponding Member of NAS U Stoika R.S., Group leader – Doctor of Chemical Sciences Zaichenko O.S.
21. "Creation of unified systems based on functional nanocarriers for the detection and isolation of pathogenic factors of biological origin" – National Academy of Sciences of Ukraine (2010-2014) – Co-leader of the project – Corresponding Member of the NAS of Ukraine Stoika R.S.
22. "New biocompatible nanocomposites with encapsulated fluorescein: their biofunctionalization and effect on cells" – National Academy of Sciences of Ukraine (2010-2014) – Project leader – Corresponding Member of the NAS of Ukraine Stoika R.S., Group leader – Doctor of Chemical Sciences Zaichenko O.S.
23. "Antitumor effect of chemotherapeutic drugs when delivered by new nanocomposite carriers functionalized with N-acylethaneamines" – National Academy of Sciences of Ukraine (2010-2014) – Co-leader of the project – Corresponding Member of the NAS of Ukraine Stoika R.S., group leader – Doctor of Chemical Sciences Zaichenko O.S., executor – Doctor of Biological Sciences Panchuk R.R.
24. "Mechanisms of action in vitro and in vivo of new compounds with proapoptotic activity, ways to increase the effectiveness of their antineoplastic action and justification of new approaches in the diagnosis and assessment of the development of autoimmune and hemato-oncological diseases" – NAS of Ukraine, state registration number 0112U002174 (2012-2016) – supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
25. "Improving the efficiency of target gene delivery to plants using new nanosized polycationic polymers" - NAS of Ukraine, grant of the targeted complex interdisciplinary scientific research program of NAS of Ukraine "Molecular and cellular biotechnologies for the needs of medicine, industry and agriculture" – 2017-2019 supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
26. "Target gene delivery into mammalian cells using novel native biocompatible polymeric nanomaterials" – Program-targeted and competitive topics of the NAS of Ukraine - Grants of the NAS of Ukraine to research laboratories/groups of young scientists of the NAS of Ukraine for conducting research in priority areas of science and technology development 2020-2021, state registration number 0120U100197 (2020-2021) – supervisor - Finiuk N.S., Ph.D.
27. "Interrelations between immunomodulatory and antitumor actions of new heterocyclic compounds (thiazole and 4-thiazolidinone derivatives)". Program-targeted and competitive topics of the NAS of Ukraine, state registration number 0120U103077 (2020-2022) – supervisor – Dr.Sc. Lootsyk M.D.
28. "Novel Dual Inhibitors of Casein Kinase 2 and Histone Deacetylase for Targeted Tumor Chemotherapy" - NAS of Ukraine, grant to research groups of young scientists of NAS of Ukraine for conducting research in priority areas of development of science and technology 2021-2022, state registration number 0121U110732 (2021-2022) – Scientific supervisor – Dr.Sc. Panchuk R.R.
29. Departmental topic "The role of immunomodulatory in the antitumor action of traditional and experimental medicines at their delivery by polymeric nanocarriers to various target cells in vitro and in vivo" – state budget topic of the NAS of Ukraine, state registration number 0122U002240 (2022-2026) – supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
30. "Enhancement of biological activity of berberine-like derivatives via their covalent and non-covalent modifications" - NAS of Ukraine, under the budget program "Support for the development of priority areas of scientific research" – OK 0222U005151 (2022) – supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
31. "Anti-inflammatory and wound-healing agent containing the composition of the cannabimimetic N-stearoylethanolamine with chitosan hydrogel" – Grant of the National Research Foundation of Ukraine, competition "Science for the reconstruction of Ukraine in the war and post-war periods", registration number 2022.01/0207, state registration number 0123U103497 (2023-2024) – supervisor – Corresponding Member of the NAS of Ukraine Stoika R.S.
32. "Molecular design, synthesis and preclinical testing of new thiazole and thiazolidine derivatives with selective action on cells of myeloproliferative neoplasms" – Grant of the National Research Foundation of Ukraine, competition "Advanced Science in Ukraine", registration number 2023.03/0104, state registration number 0124U003835 (2024-2026) – supervisor (PI) – Finiuk N.S., Ph.D.

Institute of Cell Biology National Academy of Sciences of Ukraine

 

Department of Regulation of Cell Proliferation and Apoptosis

 

Personnel:

 

• Rostyslav Stoika – Head of the Department, D. Sci. (Biol.), Professor, Corresponding Member of NAS of Ukraine;
• Maxim Lutsik – Senior Scientist, Dr.Sci. (Biol.), Professor;
• Rostysav Panchuk – Senior Researcher, Dr.Sci. (Biol.);
• Volodymyr Antonyuk – Senior Researcher, Dr.Sci. (Pharm.), Professor;
• Nataliya Finiuk – Researcher, PhD;
• Julia Senkiv – Researcher, PhD;
• Nataliya Kashchak – Junior Research Scientist, PhD;
• Yuliia Kozak – Junior Research Scientist, PhD;
• Nazar Manko – Junior Research Scientist, PhD;
• Iryna Ivasechko – Junior Research Scientist, PhD;
• Nadiya Skorohid – Lead Engineer, PhD;
• Olha Klyuchivska – Lead Engineer;
• Mykola Klishch – Postgraduate student.

 

Laboratory

 

Laboratory of the Mechanisms of Intercellular Interaction, Head – Nataliya Finiuk, PhD

Institute of Cell Biology National Academy of Sciences of Ukraine

 

Department of Cell Signaling

 

Head of Department – Oleh STASYK, PhD, D.Sc., Senior Researcher.

 

Department of Cell Signaling (the name was approved by the Academic Council of the Institute of Biochemistry of the NAS of Ukraine on October 19th, 2000) is the successor of the Department of Biochemistry of Cell Differentiation, established in 1969 as part of the O.V. Palladin Institute of Biochemistry of the AS of Ukraine, since 1974 as a part of the Lviv Department of the O.V. Palladin Institute of Biochemistry of the AS of Ukraine, in 1992 renamed as the Department of Cell Regulatory Systems of the O.V. Palladin Institute of Biochemistry of the NAS of Ukraine. From the moment of its establishment until 1998, the department was headed by the Honored Worker of Science and Technology of Ukraine, Professor Stepan Kusen, and from 1999 to 2006, the head of the department (since 2000 as part of the newly established Institute of Cell Biology of the NAS of Ukraine) was Dr. Lyudmyla Drobot.

 

Department of Cell Signaling (DCS) employs: 1 leading researcher (0.5 part-time position), 3 junior researchers, 1 graduate student and 1 engineer. Since 2007 the employees have defended 5 PhD theses (Vynnytska-Myronovska B., Kurlishchuk Y., Pavlyk Y., Karatsai O., Chen O.) and 1 D.Sc. dissertation (Stasyk O.).

 

DCS conducts international-level research on the development of new approaches to metabolic therapy of malignant tumors based on amino acid starvation. A number of new combined approaches have been proposed, which should make the corresponding therapy more effective and selective. In particular, it was established for the first time in studies conducted during 2007-2025 on a wide range of cell models that:

1. the level of endogenous enzymes of arginine amino acid biosynthesis in tumor cells of different types does not determine their sensitivity to arginine starvation in vitro in monolayer culture or 3D spheroid culture;
2. the sensitivity of malignant cells to starvation for individual amino acids is significantly reduced in 3D cultures compared to 2D cultures;
3. arginine deficiency selectively and significantly inhibits the motility, invasive and clonogenic potential of different types of tumors;
4. inhibitors of autophagy, proteasomal degradation, inducers of oxidative stress, and inducers of endoplasmic reticulum stress enhance the effect of monotherapy based on arginine deficiency and radiosensitize a number of tumor types;
5. the plant-derived arginine analogues, canavanine and indospicine, significantly and selectively enhance the proapoptotic potential of metabolic therapy based on arginine deprivation, causing acute endoplasmic reticulum stress and deregulation of a number of cell signaling mechanisms, in particular, the mTOR and MAPK pathways;
6. indospicine, which accumulates in the biomass of the subtropical legume plant Indigofera spicata, is not subject to degradation by recombinant enzymes-hydrolases human arginase I and bacterial arginine deiminase, which are used in metabolic therapy in clinics.

Testing of combination therapy approaches developed on in vitro cultured cell models in laboratory mouse models with transplanted tumors is in progress.

 

Since 2021, the Department has been actively and successfully developing an applied studies on obtaining monoclonal antibodies to protein-antigens of biotechnological and medical importance using hybridoma technology, which resulted in the implementation of the antibodies obtained in the DCS as part of the rapid lateral flow tests of the company "ХEMA" for the diagnosis of coronavirus (2021) and tuberculosis (2024) pathogens by immunochromatographic analysis, as evidenced by the corresponding acts of implementation.

 

DCS has created a new model of neurodegenerative Parkinson's disease based on recombinant strains of the yeast Ogataea polymorpha, expressing the cytotoxic human alpha-synuclein protein, which is involved in the pathogenesis of this disease. Research has begun on studying the effects of autophagy modulation, exogenous polyamines, metal ions, oxidative stress and other factors on the cytotoxicity of the corresponding protein.

 

In recent years, the Department has initiated research aimed at identifying new informative markers related to the diagnosis, prognosis and monitoring of patients with traumatic brain injury (TBI). A bank of blood plasma samples from patients with TBI is being created. The aim of this work is to create prototypes of rapid tests for TBI that can be used in frontline regions with disrupted medical infrastructure, by military medics and in sports medicine.

 

The Department is equipped with boxes for working with microorganisms and human and animal in vitro cell cultures. DCS also has its own cryobank of pseudonormal and malignantly transformed human and animal cell lines and a collection of model strains of microorganisms (bacteria, yeasts). DCS has its own modernized animal facility, which houses C57Bl/6 and BALB/c mice, allowing studies of the toxicity of various compounds, the effectiveness of antitumor therapy in vivo, and the production of monoclonal antibodies in animals. In recent years, the Department has undergone a significant upgrade to its equipment. From several sources, in particular, due to the funding of the project No. 2023.04/0048 of the National Research Fund of Ukraine, biomedical boxes for sterile work, an automatic hematological analyzer, an inverted microscope, a microplate photometer, laboratory centrifuges, a PCR machine, etc., have been purchased and actively exploited.

Institute of Cell Biology National Academy of Sciences of Ukraine

 

Department of Cell Signaling

 

The main scientific interests of the Department are:

 

• Search for new approaches to metabolic anticancer therapy, in particular, based on artificial starvation for individual amino acids and combined approaches;


• Research on natural analogues of amino acids as potential antitumor and antimicrobial agents;


• Development of new model of Parkinson's disease based on methylotrophic yeasts;


• Designing stable hybridoma clones and monoclonal antibodies against protein-antigens of biotechnological and medical importance to develop new diagnostic systems.

Institute of Cell Biology National Academy of Sciences of Ukraine

 

Department of Cell Signaling

 

Publications

 

Year	Published scientific articles		Total impact factor
	All	Including articles in the international journals
2008	3		___	View publications
2009	5		___	View publications
2010	3		___	View publications
2011	11		12.795	View publications
2012	5		13.262	View publications
2013	4		7.464	View publications
2014	5		6.876	View publications
2015	4		8.659	View publications
2016	8	3	12.300	View publications
2017	4	3	13.940	View publications
2018	5	5	17.590	View publications
2019	2	1	10.659	View publications
2020	6	5	22.000	View publications
2021	5	4	30.700	View publications
2022	1		___	View publication
2023	___		___
2024	4	1	4.500	View publication
2025	3	2	8.800	View publication

2008

 

1. Stasyk O.G., Maidan M., Stasyk O.V., Van Dijck P., Thevelein J.M., Sibirny A.A. Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha // Eukaryotic Cell. -2008.- V.7, № 4. – P.735-746.
2. Ishchuk O.P., Voronovsky A.Y., Stasyk O.V., Gayda G.Z., Gonchar M.V., Abbas C.A., Sibirny A.A. Improvement of xylose high-temperature fermentation in Hansenula polymorpha due to overexpression of the PDC1 gene coding for pyruvate decarboxylase // FEMS Yeast Research. – 2008. – V.8, № 7. – P.1164-1174.
3. Sybirna N., Brodiak I., Ferents I., Barska M. The effect of L-arginine on the oxidative modification of plasma fibrinogen under experimental diabetes mellitus in rats // Annal. Univers. Mariae Curie-Sklodovska, sectio AA Biologia – 2008. – V. IX,– P. 115-118.

2009

 

1. Havrylov S., Rzhepetskyy Yu., Malinowska A., Drobot L., Rendowicz M.J. Proteins recruited by SH3 domains of Ruk/CIN85 adaptor identified by LC-MS/MS // Proteome Science.-2009.-Vol. 7, N21.-P. 1-19.
2. O.I. Basaraba, M.L. Barska, Ya.P. Bobak, H.Yu. Shuvayeva, S.M. Marchenko, V. R. Savran, N.A. Volodko, V.L. Buchman, L.B. Drobot. Expression of (RUK/CIN85)/(CD2AP/CMS) family adapter proteins in human breast adenocarcinoma // Clin. and Experim. Potol.-2009- V. 9, № 4. – P. 125-130.
3. Rzhepetskyy Y.А., Svinchuk V.V., Buchman V.L.,Drobot L.B. Comparative analysis of Ruk/CIN85 phosphorylation in rat pheochromocytome cell line PC12 under growth factors stimulation// Clinical and eksperimental pathology. 2009.- V. VIII, №2.- p. 64-67.
4. Brodyak I.V., Zdioruk N.І., Barska M.L., Vovk O.І., Sybirna N.O. Lectincytochemical analysis of mononuclear leukocyte plasmatic membrane sialocontaining glycoprotein of peripheral blood under type 1 diabetes mellitus // Med.Chim. – 2009. – № 4. – P. 76–79 (Ukrainian).
5. Zdioruk M.I., Barska M.L., Brodyak I.V., Vovk O.I. Urbanovych A.M., Sybirna N.O. Influence of wortmannin on aggregation ability of neutrophylic granulocytes under type 1 diabetes mellitus / Studia Biologica.– 2009. –V.3, №2. – P. 133–140 (Ukrainian).

2010

 

1. Bobak Y.P., Vynnytska B.O., Kurlishchuk Y.V., Sibirny A.A., Stasyk O.V. Cancer cell sensitivity to arginine deprivation in vitro is not determined by endogenous levels of arginine metabolic enzymes // Cell Biol Int.-2010.-V.34 (11).-P.1085–1089.
2. Stadnyk V.V., Izyumovaa L.A., Rzhepetskiy Y.A., Mayor C.Y., Vlizlo V.V. The Antisense Oligonucleotides Decrease Expression of the Cellular Prion // Letters in Drug Design & Discovery.- 2010,-V.7.- P.23-26.
3. Jóźwiak J., Rzhepetskiy Y., Sobczak M., Kocik E., Skórzewski R., Kłopocka W., Rędowicz M.J. Amebin a novel Amoeba Proteus actin-bundling protein inhibits actomyosin ATPase activity and is essential for Amoeba migration // Archives of Biochemistry and Biophysics.- 2010, (published ahead of print).

 

Tutorials and methodical editions:

1. Chemistry of the Protein: texbook: [for university students] / [ N.О. Sybyrna, М.V. Gonchar, І.V. Brodyak, О.G. Stasyk, М.L. Barska] edited by Prof. N.О. Sybyrna. - Lviv: Ivan Franko Lviv national University. 2010 - pp.393 . Labeled by MES of Ukraine.

2011

 

1. Vynnytska B.O., Mayevska O.M., Kurlishchuk Y.V., Bobak Y.P., Stasyk O.V. Canavanine augments proapoptotic effects of arginine deprivation in cultured human cancer cells // Anticancer Drugs. – 2011. – V.22 (2). – P. 148 – 157. (IF=2.407) (Scopus)
2. Jozwiak J, Rzhepetskyy Y, Sobczak M, Kocik E, Skorzewski R, Klopocka W, Redowicz MJ. C-terminal fragment of amebin promotes actin filament bundling, inhibits acto-myosin ATPase activity and is essential for amoeba migration // Arch. Biochem. Biophys. – 2011. – V. 506(1). – P. 99-108. (IF=2.935) (Scopus)
3. Nazarko V.Y., Nazarko T.Y., Farre J.C., Stasyk O.V., Warnecke D., Ulaszewski S., Cregg J.M., Sibirny A.A., Subramani S. Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris // Autophagy. – 2011. – V. 7(4). – P. 375-385. (IF=7.453) (Scopus)
4. Sybirna N., Brodyak I., Zdioruk M., Barska M. Sialic acid-containing glycoproteins are the marker molecules that determine the leukocyte functional state under diabetes mellitus // Sepsis. 2011. – V. 4(1) – P. 47-55. (Google Scholar)
5. Стасюк Н.Є., Карпʼяк В.В., Гайда Г.З., Гайда А.В., Стасик О.В., Гончар М.В. Синтез афінних сорбентів для виділення рекомбінантної аргінази і людини із генетично модифікованих дріжджів Нansenula polymorpha //Праці НТШ, Хем. Біохем. – 2011. – Т. 28. – С.139-149. (Google Scholar)
6. O.I. Chen, L.S. Lyniv, N.I. Igumentseva, M.L. Barska, N.O. Effect of nitric oxide donor on viability of human leukemic cells upon arginine deprivation // Studia Biologica. – 2011. – V. 5, N 2. – P. 17–28. (Google Scholar)
7. Сибірна Н.О., Здіорук М.І., Бродяк І.В., Барська М.Л., Вовк О.І. Активація фосфатидилінозитол-3′-кіназного шляху лектиніндукованим сигналом через сіаловмісні глікопротеїни мембран лейкоцитів при цукровому діабеті 1-го типу // Укр. біохім. Журнал. – 2011. – Т. 83. - № 5. – С. 22-31. (Google Scholar)
8. Vovk O.I., Rzhepetskyy Y.A., Pereverzeva H.G., Stasyk O.V. Expression of recombinant human argininosuccinate synthetase in Escherichia coli // Біологічні Студії / Studia Biologica. – 2011. – Том 5. - №3 - С. 19–28. (Google Scholar)
9. Стасюк Н., Гайда Г., Гайда А., Стасик О., Карпʼяк В., Гончар М. Синтез афінних сорбентів для очищення аргінази І людини із рекомбінантних дріжджів Нansenula polymorpha // Праці НТШ, Хем. Біохем. – 2011. – Т.28. – С.139-149. (Google Scholar)
10. Стасик О. Г., Стасик О. В. Селективна деградація пероксисом у дріжджів Hansenula polymorpha потребує стеролглюкозилтрансферазу Atg26 // Біологічні студії. – 2011. – Т.5, №2. – С. 93-104. (Google Scholar)
11. Ю.В. Курліщук, Б.О. Винницька-Мироновська, Я.П. Бобак, О.В. Стасик (2011) Вплив метаболітів аргініну на життєздатність людських пухлинних клітин за умов дефіциту цієї амінокислоти in vitro. Біологічні студії. – 2011. - Т.5, №2. - С. 5-16. (Google Scholar)

2012

 

1. Vynnytska-Myronovska B., Bobak Y., Garbe Y., Dittfeld C., Stasyk O., Kunz-Schughart L.A. Single amino acid arginine starvation efficiently sensitizes cancer cells to canavanine treatment and irradiation // Int. J. Cancer. – 2012. – V. 130, № 9. – P. 2164 – 2175 (IF=6.198). (Scopus)
2. Zakalskiy AE, Zakalska OM, Rzhepetskyy YA, Potocka N, Stasyk OV, Horak D, Gonchar MV. Overexpression of (His)(6)-tagged human arginase I in Saccharomyces cerevisiae and enzyme purification using metal affinity chromatography // Protein Expr Purif. -2012.- 81(1). –P. 63-68. (IF=1.429) (Scopus)
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