Institute of Cell Biology National Academy of Sciences of Ukraine

Institute of Cell Biology National Academy of Sciences of Ukraine

Institute of Cell Biology National Academy of Sciences of Ukraine

Сooperation with stakeholders

Cooperation agreements, minutes of meetings

• University of Rzeszów
• Politechnika Krakowska im. Tadeusza Kościuszki (Kraków, Polska)
• Institute of Cancer Research Department of Medicine I (Vienna, Austria)
• Instytut Biologii Doświadczalnej im. M. Nenckiego PAN (Warszawa, Polska)
• Lviv State University of Physical Culture (Lviv, Ukraine)
• D.K. Zabolotny Institute of Microbiology and Virology of the NASU (Kyiv, Ukraine)
• Biology Institute, Shandong Academy of Sciences (Jinan, China)
• Ivano-Frankivsk National Medical University
• ДУ «Інститут патології крові та трансфузійної медицини НАМН України» (Львів, Україна)
• Ivan Franko National University of Lviv
• Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv
• Guizhou Medical University
• EXPLOGEN LLC

Meeting with a stakeholder:

• Ivan Franko National University of Lviv
• EXPLOGEN LLC

Employment prospects

Who can I work for?

Stakeholders answers

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Molecular Genetics and Biotechnology

Head of the Department – ​​Andriy Andriyovych SIBIRNY, Doctor of Biological Sciences, Professor, Academician of the National Academy of Sciences of Ukraine.

The department was founded in 1988 on the basis of the Laboratory of Biochemical Genetics, which was established in 1985 and headed by Andriy Andriyovych Sibirny. He is a laureate of the O. Palladin Prize of the NAS of Ukraine in the field of biochemistry (2004) and the I. Mechnikov Prize of the NAS of Ukraine in the field of microbiology (2014). He also received the honorary title of "Honored Worker of Science and Technology of Ukraine" (2008), is a foreign member of the Latvian Academy of Sciences and a laureate of the State Prize of Ukraine in the field of science and technology (2012).

A. Sibirny is the author of more than 300 scientific articles, including 34 chapters in monographs, more than 40 patents, several hundred abstracts of oral and poster presentations, including more than 40 plenary lectures, at domestic and international conferences. His Hirsch index is 38 (Scopus), the total impact factor of publications (Scopus) is over 300, the total number of citations is over 15000 (Scopus). Co-author of the monograph "Microbial synthesis of flavinins" ("Naukova dumka", 2006). Chief Editor of monographs: "Living organisms and bioanalytical approaches for detoxication and monitoring of toxic compounds" (University of Rzeszow, 2015); "Biotechnology of yeasts and filamentous fungi" (Springer, 2017); "Non-Conventiona Yeasts: from Basic Research to Application" (Springer Nature, 2019); "Flavins and Flavoproteins" (Springer Nature, 2021); "Biotechnology of Yeasts and Filamentous Fungi. Grand Challenges in Biology and Biotechnology" (Springer, 2025).

Academician A.A. Sibirny was the Chairman (2008-2012), and is currently a member of the International Commission on Yeasts of the International Association of Microbiological Societies, a member of the International Committee on Molecular Biology and Genetics of Yeasts, a delegate to the International Federation of Cell Biology (IFCB), was the Director of FEMS for Microbiological Education and Promotion of Microbiological Achievements (2018-2024).

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Molecular Genetics and Biotechnology

The main areas of scientific research in the department:

1. Mechanisms of degradation of peroxisomal and cytosolic proteins in yeasts.


2. Regulation of flavinogenesis in non-conventional yeast. Cloning of structural and regulatory genes involved in biosynthesis of riboflavin and its derivatives (flavin coenzymes and antibiotics).


3. Production of biofuel ethanol by fermentation of lignocellulosic hydrolysates by non-conventional yeasts.


4. Identification of mechanisms of functioning genes and their products involved in the metabolism and alcoholic fermentation of pentose sugars (xylose, L-arabinose) in thermotolerant methylotrophic yeast.


5. Development of a vaccine against SARS-CoV-2 based on the expression of S protein fragments in yeast and the study of the immune response in laboratory animals.

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Molecular Genetics and Biotechnology

Publications

2008

1. Dmytruk O. V., Dmytruk K. V., Abbas C. A., Voronovsky A. Y., Sibirny A. A. Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha // Microb. Cell. Fact. - 2008. – №. 7. - P. 21.
2. Dmytruk O. V., Voronovsky A. Y., Abbas C. A., Dmytruk K. V., Ishchuk O. P., Sibirny A. A. Overexpression of bacterial xylose isomerase and own xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha // FEMS Yeast Res. – 2008. – Vol. 8, P. 165-173.
3. Ishchuk O. P., Dmytruk K. V., Rohulya O. V., Voronovsky A. Y., Abbas C. A., Sibirny A. A. Development Of A Promoter Assay System For The Flavinogenic Yeast Candida famata Based On The Kluyveromyces Lactis ß-Galactosidase Lac4 Reporter Gene. // Enzyme Microbial Technology. – 2008. – Vol. 42,N3. – P. 208-215.
4. Ishchuk O. P., Voronovsky A. Y., Stasyk O. V., Gayda G. Z., Gonchar M. V., Abbas C. A., Sibirny AA. Overexpression of pyruvate decarboxylase in the yeast Hansenula polymorpha results in increased ethanol yield in high-temperature fermentation of xylose. // FEMS Yeast Res. – 2008. – Vol. 8. – P. 1167-1174.
5. Ksheminska H., Fedorovych D., Honchar T., Ivash M., Gonchar M. Yeast tolerance to chromium depends on extra-cellular chromate reduction and Cr(III)-chelation // Food Technol. Biotechnol. - 2008. - Vol.46, №4. – P.420-427.
6. Lahtchev K. L., Batovska D. I., Parushev S. P., Ubiyvovk V. M., Sibirny A. A. Antifungal activity of chalcones: A mechanistic study using various yeast strains // Eur. J. Med. Chem. – 2008. - V.43, №10. - P. 2220-2228.
7. Nazarko V. Y., Thevelein J. M., Sibirny A. A. G-protein-coupled receptor Gpr1 and G-protein Gpa2 of cAMP-dependent signaling pathway are involved in glucose-induced pexophagy in the yeast Saccharomyces cerevisiae // Cell Biol. Int. – 2008. - № 32. – P. 502- 504.
8. Nazarko V. Y., Futej K. O., Thevelein J. M., Sibirny A. A. Differences in glucose sensing and signaling for pexophagy between the baker’s yeast Saccharomyces cerevisiae and the methylotrophic yeast Pichia pastoris // Autophagy. – 2008. - № 4. – P. 381- 384.
9. Dmitruk KV, Smutok OV, Gonchar MV, Sibirnyĭ AA. Construction of flavocytochrome b2-overproducing strains of the thermotolerant methylotrophic yeast Hansenula polymorpha (Pichia angusta) // Mikrobiologia (Moscow). – 2008. - V.77. – P.213-218. (in Russian).
10. Dmytruk O.V., Dmytruk K.V., Voronovsky A.Y., Sibirny A.A. Metabolic engineering of the initial stages of xylose catabolism in yeasts for construction of efficient producers of ethanol from lignocelluloses // Tsitologiia i genetika (Cytology and Genetics) – 2008. – Vol..42. – P.127-138.
11. Sibirny V.А., Gonchar М.V., Grabek-Lejko D., Pavlishko H.М., Csöregi E., Sibirny A.A. Photometric assay of methanol and formaldehyde in industrial waste-waters using alcohol oxidase and 3-methyl-2-benzothiazolinone hydrazone // Intern. J. Environ. Anal. Chem. – 2008. – V. 88, N 4. – P. 289-301.
12. Sibirny A. A., Voronovsky A. Y. Chapter 5: Candida famata (Debaryomyces hansenii). In: Yeast biotechnology: diversity and applications (G. Kunze, T. Satyanarayana Eds), Springer Science. 2008; Р. 83-110.
13. Sibirny А.А, Boretsky Y.R. Chapter 6: Pichia guilliermondii. In: Yeast biotechnology: diversity and applications (G. Kunze, T. Satyanarayana Eds), Springer Science. 2008; РР 111-132.
14. Klionsky D.J. et al. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes (Review)// Autophagy. – V.4, №2. – P.151-175.
15. Stasyk O.G., Maidan M. M., Stasyk O. V., Van Dijck P., Thevelein J.M., Sibirny A.A. Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha. Eukaryotic cell. – 2008. – Vol.7(4). – P.735-746.

2009

1. Yatsyshyn, V.Y., Fedorovych, D.V., Sibirny, A.A. The microbial synthesis of flavin nucleotides: a review // Appl. Biochem. Microbiol. – 2009. – Vol. 45, No 2. – Р. 115–124.
2. Pynyaha YV, Boretsky YR, Fedorovych DV, Fayura LR, Levkiv AI, Ubiyvovk VM, Protchenko OV, Philpott CC, Sibirny AA. Deficiency in frataxin homologue YFH1 in the yeast Pichia guilliermondii leads to missregulation of iron acquisition and riboflavin biosynthesis and affects sulfate assimilation // Biometals. – 2009. – Vol.22. - P.1051-1061.
3. Cheon SA, Choo J, Ubiyvovk VM, Park JN, Kim MW, Oh DB, Kwon O, Sibirny AA, Kim JY, Kang HA. New selectable host-marker systems for multiple genetic manipulations based on TRP1, MET2 and ADE2 in the methylotrophic yeast Hansenula polymorpha // Yeast. – 2009. – Vol. 26, N9. - P.507- 521.
4. Voronovsky AY, Rohulya OV, Abbas CA, Sibirny AA. Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan. // Metabolic Engineering. – 2009. – Vol. 11, N4-5. – P. 234-242.
5. Ishchuk OP, Voronovsky AY, Abbas CA, Sibirny AA. Construction of Hansenula polymorpha strains with improved thermotolerance // Biotechnol. Bioeng. – 2009. – Vol.104, N5. – P. 911-919.
6. Yatsyshyn VY, Ishchuk OP, Voronovsky AY, Fedorovych DV, Sibirny AA. Production of flavin mononucleotide by metabolically engineered yeast Candida famata. // Metabolic Engineering. – 2009. Vol.11, N3. – P. 163-167.
7. Shkil H, Stoica L, Dmytruk K, Smutok O, Gonchar M, Sibirny A, Schuhmann W. Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b(2) // Bioelectrochemistry. 2009, 76(1-2):175-9
8. Fedorovych D., Gonchar M., Ksheminska H., Prokopiv T., Nechay H., Kaszycki P., Koloczek H., Sibirny A. Mechanisms of chromate detoxification in yeasts // Miсrobiology and Biotechnology. - 2009. – №.7. – С.15-21

2010

1. Bobak Y.P., Vynnytska B.O., Kurlishchuk Y.V., Sibirny A.A., Stasyk O.V. Cancer cell sensitivity to arginine deprivation in vitro is not determined by endogenous levels of arginine metabolic enzymes // Cell Biol Int. – 2010. - Vol.34, №11. - P.1085-1089.
2. Ishchuk O.P., Abbas C.A., Sibirny A.A. Heterologous expression of Saccharomyces cerevisiae MPR1 gene confers tolerance to ethanol and L: -azetidine-2-carboxylic acid in Hansenula polymorpha. J Ind. Microbiol .Biotechnol. - 2010. – Vol.37, N2. P. 213-218.
3. Dmytruk K.V., Yatsyshyn V.Y., Sibirna N.O., Fedorovych D.V., Sibirny A.A. Metabolic engineering and classic selection of the yeast Candida famata (Candida flareri) for construction of strains with enhanced riboflavin production // Metabolic Engineering. – 2011. - Vol.13. – P.33-38.
4. Yatsyshyn V.Y., Fedorovych D.V., Sibirny A.А. Medium optimization for production of flavin mononucleotide by the recombinant strain of the yeast Candida famata strain using statistical designs // Biochemical Engineering Journal. - 2010. - Vol.49, P.52-60.
5. Sybirna K, Petryk N, Zhou YF, Sibirny A, Bolotin-Fukuhara M. A novel Hansenula polymorpha transcriptional factor HpHAP4-B, able to functionally replace the S. cerevisiae HAP4 gene, contains an additional bZip motif. // Yeast. - 2010. – Vol. 27, №11. - P.941-954.
6. Polupanov A.S., Nazarko V.Y., Sibirny A.A. CCZ1, MON1 and YPT7 genes are involved in pexophagy, Cvt pathway and non-specific macroautophagy in the methylotrophic yeast Pichia pastoris. // Cell Biol. Int. - 2011. – Vol.35, N4. – P.311-319.

2011

1. Abbas C. A., Sibirny A. A. Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers // Microbiol. Mol. Biol. Rev. – 2011. - Vol.75, № 2. - Р.321-360.
2. Grabek-Lejko D., Kurylenko O. O., Sibirny V. A., Ubiyvovk V. M., Penninckx M, Sibirny A. A. Alcoholic fermentation by wild-type Hansenula polymorpha and Saccharomyces cerevisiae versus recombinant strains with an elevated level of intracellular glutathione // J. Ind. Microbiol. Biotechnol. – 2011. – Vol.38, №11. - Р. 1853-1859.
3. Millerioux Y., Clastre M., Simkin A. J., Courdavault V., Marais E., Sibirny A. A., Noël T., Crèche J., Giglioli-Guivarcʼh N., Papon N. Drug-resistant cassettes for the efficient transformation of Candida guilliermondii wild-type strains // FEMS Yeast Res. – 2011. – Vol.11, №6. – Р.457-463.
4. Dmytruk K. V., Smutok O. V., Dmytruk O. V., Schuhmann W., Sibirny A. A. Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor // BMC Biotechnol. –2011. - Vol.11.- P.58-65.
5. Boretsky Y. R., Pynyaha Y. V., Boretsky V. Y., Fedorovych D. V., Fayura L. R, Protchenko O., Philpott C. C., Sibirny A. A. Identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast Pichia guilliermondii using insertion mutagenesis // FEMS Yeast Res. – 2011. - Vol.11, №3. Р. 307-314.
6. Ubiyvovk V. M., Ananin V. M., Malyshev A. Y., Kang H. A, Sibirny A. A. Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorpha DL-1 // BMC Biotechnol. – 2011. – Vol.11. P.8-19.
7. Millerioux Y., Clastre M., Simkin A. J., Marais E., Sibirny A. A., Noël T., Crèche J., Giglioli-Guivarcʼh N., Papon N. Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain // J. Microbiol. Methods. – 2011. - Vol.84, №2. Р.355-358.
8. Nazarko V. Y., Nazarko T. Y., Farré J. C., Stasyk O. V., Warnecke D., Ulaszewski S., Cregg J. M., Sibirny A. A., Subramani S. Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris // Autophagy. – 2011. Vol.7, №4. Р. 375-385.
9. Smutok O., Broda D., Smutok H., Dmytruk K., Gonchar M. Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b(2) // Chemosphere. – 2011. - Vol.83, №4. Р.449-454.
10. Sibirny A.. Metabolic engineering of non-conventional yeasts for construction of robust producers of fuels and chemicals // Nowoczene metody analizy surowcỏw rolniczych. Rzeszỏw: PAJ-Press. – 2011. - P. 445-453.
11. Sibirny W., Smutok O., Klepach H., Gayda G., Dmytruk K., Broda D., Gonchar M. Alcohol-selective amperometric biosensors based on natural and mutated alcohol oxidases // Nowoczene metody analizy surowcỏw rolniczych. Rzeszỏw: PAJ-Press. – 2011. - P. 241-253.
12. Grabek-Lejko D., Sibirny V., Kluz M., Sibirny A. Droźdźe niekonwencjonalne jako potencjialni producenci etanolu paliwowego z lignocelulozowych odpadỏw rolniczych. // Nowoczene metody analizy surowcỏw rolniczych. Rzeszỏw: PAJ-Press. – 2011. - P. 369-378.
13. Smutok O., Gayda G., Dmytruk K., Klepach H., Nisnevitch M., Sibirny A., Puchalski C., Broda D., Schuhmann W., Gonchar M., Sibirny V. Amperometric Biosensors for Lactate, Alcohols, and Glycerol Assays in Clinical Diagnostics // Biosensors - Emerging Materials and Applications. - 2011. – 630 р.
14. Sibirny A.A. Mechanisms of autophagy and pexophagy in yeasts // Biochemistry (Mosc). – 2011. – V. 76, No. 12. – P. 1279-1290.