Institute of Cell Biology National Academy of Sciences of Ukraine

Сooperation with stakeholders

Cooperation agreements, minutes of meetings

• University of Rzeszów
• Politechnika Krakowska im. Tadeusza Kościuszki (Kraków, Polska)
• Institute of Cancer Research Department of Medicine I (Vienna, Austria)
• Instytut Biologii Doświadczalnej im. M. Nenckiego PAN (Warszawa, Polska)
• Lviv State University of Physical Culture (Lviv, Ukraine)
• D.K. Zabolotny Institute of Microbiology and Virology of the NASU (Kyiv, Ukraine)
• Biology Institute, Shandong Academy of Sciences (Jinan, China)
• Ivano-Frankivsk National Medical University
• ДУ «Інститут патології крові та трансфузійної медицини НАМН України» (Львів, Україна)
• Ivan Franko National University of Lviv
• Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv
• Guizhou Medical University
• EXPLOGEN LLC

Meeting with a stakeholder:

• Ivan Franko National University of Lviv
• EXPLOGEN LLC

Employment prospects

Who can I work for?

Stakeholders answers

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Molecular Genetics and Biotechnology

Head of the Department - Andriy Sibirny, Ph.D., Dr. Sci., Professor, Member of NAS of Ukraine.

The Department was established in 1988 on the base of the Laboratory of Biochemical Genetics which was found in 1985, and since that time it is headed by Andriy Sibirny, Dr.Biol.Sci., Professor, Member of National Academy of Sciences of Ukraine. He was awarded with O. Palladin prize of NAS of Ukraine in the field of biochemistry (2004) and I. Metchnikov prize of NAS of Ukraine in the field of microbiology (2014). He is the laureate of State award for the achievements in science and technology (2012). A. Sibirny is also honored worker of Ukraine in science and technology (2008). A. Sibirny is the author of more than 220 full-length scientific articles including three monographs and near 150 papers in peer-reviewed International scientific journals, 28 Patent Applications, 100 abstracts of oral and poster communications of conferences, including 40 plenary lectures on domestic and International scientific meetings.

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Molecular Genetics and Biotechnology

The main research topics:

• Mechanisms of degradation of peroxisomal and cytosolic proteins in yeasts.


• Regulation of flavinogenesis in nonconventional yeasts. Cloning of structural and regulatory genes involved in biosynthesis of riboflavin and its derivatives (flavin coenzymes, roseoflavin) and its.


• Role of glutathione (GSH) in stress response of methylotrophic yeast cells: cloning of structural and regulatory genes, construction of GSH-overproducing strains, bioaccumulation of heavy metals.


• Production of biofuel ethanol by fermentation of lignocellulosic hydrolysates by non-conventional yeasts.


• Construction of microbial strains capable to produce isobutanol (yeasts) and hydrogen (bacteria).


• Biotechnological production of proteins important for medicine and diagnostics.


• Development of new combinatorial approaches for cancer treatment.

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Molecular Genetics and Biotechnology

Publications

2008

1. Dmytruk O. V., Dmytruk K. V., Abbas C. A., Voronovsky A. Y., Sibirny A. A. Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha // Microb. Cell. Fact. - 2008. – №. 7. - P. 21.
2. Dmytruk O. V., Voronovsky A. Y., Abbas C. A., Dmytruk K. V., Ishchuk O. P., Sibirny A. A. Overexpression of bacterial xylose isomerase and own xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha // FEMS Yeast Res. – 2008. – Vol. 8, P. 165-173.
3. Ishchuk O. P., Dmytruk K. V., Rohulya O. V., Voronovsky A. Y., Abbas C. A., Sibirny A. A. Development Of A Promoter Assay System For The Flavinogenic Yeast Candida famata Based On The Kluyveromyces Lactis ß-Galactosidase Lac4 Reporter Gene. // Enzyme Microbial Technology. – 2008. – Vol. 42,N3. – P. 208-215.
4. Ishchuk O. P., Voronovsky A. Y., Stasyk O. V., Gayda G. Z., Gonchar M. V., Abbas C. A., Sibirny AA. Overexpression of pyruvate decarboxylase in the yeast Hansenula polymorpha results in increased ethanol yield in high-temperature fermentation of xylose. // FEMS Yeast Res. – 2008. – Vol. 8. – P. 1167-1174.
5. Ksheminska H., Fedorovych D., Honchar T., Ivash M., Gonchar M. Yeast tolerance to chromium depends on extra-cellular chromate reduction and Cr(III)-chelation // Food Technol. Biotechnol. - 2008. - Vol.46, №4. – P.420-427.
6. Lahtchev K. L., Batovska D. I., Parushev S. P., Ubiyvovk V. M., Sibirny A. A. Antifungal activity of chalcones: A mechanistic study using various yeast strains // Eur. J. Med. Chem. – 2008. - V.43, №10. - P. 2220-2228.
7. Nazarko V. Y., Thevelein J. M., Sibirny A. A. G-protein-coupled receptor Gpr1 and G-protein Gpa2 of cAMP-dependent signaling pathway are involved in glucose-induced pexophagy in the yeast Saccharomyces cerevisiae // Cell Biol. Int. – 2008. - № 32. – P. 502- 504.
8. Nazarko V. Y., Futej K. O., Thevelein J. M., Sibirny A. A. Differences in glucose sensing and signaling for pexophagy between the baker’s yeast Saccharomyces cerevisiae and the methylotrophic yeast Pichia pastoris // Autophagy. – 2008. - № 4. – P. 381- 384.
9. Dmitruk KV, Smutok OV, Gonchar MV, Sibirnyĭ AA. Construction of flavocytochrome b2-overproducing strains of the thermotolerant methylotrophic yeast Hansenula polymorpha (Pichia angusta) // Mikrobiologia (Moscow). – 2008. - V.77. – P.213-218. (in Russian).
10. Dmytruk O.V., Dmytruk K.V., Voronovsky A.Y., Sibirny A.A. Metabolic engineering of the initial stages of xylose catabolism in yeasts for construction of efficient producers of ethanol from lignocelluloses // Tsitologiia i genetika (Cytology and Genetics) – 2008. – Vol..42. – P.127-138.
11. Sibirny V.А., Gonchar М.V., Grabek-Lejko D., Pavlishko H.М., Csöregi E., Sibirny A.A. Photometric assay of methanol and formaldehyde in industrial waste-waters using alcohol oxidase and 3-methyl-2-benzothiazolinone hydrazone // Intern. J. Environ. Anal. Chem. – 2008. – V. 88, N 4. – P. 289-301.
12. Sibirny A. A., Voronovsky A. Y. Chapter 5: Candida famata (Debaryomyces hansenii). In: Yeast biotechnology: diversity and applications (G. Kunze, T. Satyanarayana Eds), Springer Science. 2008; Р. 83-110.
13. Sibirny А.А, Boretsky Y.R. Chapter 6: Pichia guilliermondii. In: Yeast biotechnology: diversity and applications (G. Kunze, T. Satyanarayana Eds), Springer Science. 2008; РР 111-132.
14. Klionsky D.J. et al. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes (Review)// Autophagy. – V.4, №2. – P.151-175.
15. Stasyk O.G., Maidan M. M., Stasyk O. V., Van Dijck P., Thevelein J.M., Sibirny A.A. Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha. Eukaryotic cell. – 2008. – Vol.7(4). – P.735-746.

2009

1. Yatsyshyn, V.Y., Fedorovych, D.V., Sibirny, A.A. The microbial synthesis of flavin nucleotides: a review // Appl. Biochem. Microbiol. – 2009. – Vol. 45, No 2. – Р. 115–124.
2. Pynyaha YV, Boretsky YR, Fedorovych DV, Fayura LR, Levkiv AI, Ubiyvovk VM, Protchenko OV, Philpott CC, Sibirny AA. Deficiency in frataxin homologue YFH1 in the yeast Pichia guilliermondii leads to missregulation of iron acquisition and riboflavin biosynthesis and affects sulfate assimilation // Biometals. – 2009. – Vol.22. - P.1051-1061.
3. Cheon SA, Choo J, Ubiyvovk VM, Park JN, Kim MW, Oh DB, Kwon O, Sibirny AA, Kim JY, Kang HA. New selectable host-marker systems for multiple genetic manipulations based on TRP1, MET2 and ADE2 in the methylotrophic yeast Hansenula polymorpha // Yeast. – 2009. – Vol. 26, N9. - P.507- 521.
4. Voronovsky AY, Rohulya OV, Abbas CA, Sibirny AA. Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan. // Metabolic Engineering. – 2009. – Vol. 11, N4-5. – P. 234-242.
5. Ishchuk OP, Voronovsky AY, Abbas CA, Sibirny AA. Construction of Hansenula polymorpha strains with improved thermotolerance // Biotechnol. Bioeng. – 2009. – Vol.104, N5. – P. 911-919.
6. Yatsyshyn VY, Ishchuk OP, Voronovsky AY, Fedorovych DV, Sibirny AA. Production of flavin mononucleotide by metabolically engineered yeast Candida famata. // Metabolic Engineering. – 2009. Vol.11, N3. – P. 163-167.
7. Shkil H, Stoica L, Dmytruk K, Smutok O, Gonchar M, Sibirny A, Schuhmann W. Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b(2) // Bioelectrochemistry. 2009, 76(1-2):175-9
8. Fedorovych D., Gonchar M., Ksheminska H., Prokopiv T., Nechay H., Kaszycki P., Koloczek H., Sibirny A. Mechanisms of chromate detoxification in yeasts // Miсrobiology and Biotechnology. - 2009. – №.7. – С.15-21

2010

1. Bobak Y.P., Vynnytska B.O., Kurlishchuk Y.V., Sibirny A.A., Stasyk O.V. Cancer cell sensitivity to arginine deprivation in vitro is not determined by endogenous levels of arginine metabolic enzymes // Cell Biol Int. – 2010. - Vol.34, №11. - P.1085-1089.
2. Ishchuk O.P., Abbas C.A., Sibirny A.A. Heterologous expression of Saccharomyces cerevisiae MPR1 gene confers tolerance to ethanol and L: -azetidine-2-carboxylic acid in Hansenula polymorpha. J Ind. Microbiol .Biotechnol. - 2010. – Vol.37, N2. P. 213-218.
3. Dmytruk K.V., Yatsyshyn V.Y., Sibirna N.O., Fedorovych D.V., Sibirny A.A. Metabolic engineering and classic selection of the yeast Candida famata (Candida flareri) for construction of strains with enhanced riboflavin production // Metabolic Engineering. – 2011. - Vol.13. – P.33-38.
4. Yatsyshyn V.Y., Fedorovych D.V., Sibirny A.А. Medium optimization for production of flavin mononucleotide by the recombinant strain of the yeast Candida famata strain using statistical designs // Biochemical Engineering Journal. - 2010. - Vol.49, P.52-60.
5. Sybirna K, Petryk N, Zhou YF, Sibirny A, Bolotin-Fukuhara M. A novel Hansenula polymorpha transcriptional factor HpHAP4-B, able to functionally replace the S. cerevisiae HAP4 gene, contains an additional bZip motif. // Yeast. - 2010. – Vol. 27, №11. - P.941-954.
6. Polupanov A.S., Nazarko V.Y., Sibirny A.A. CCZ1, MON1 and YPT7 genes are involved in pexophagy, Cvt pathway and non-specific macroautophagy in the methylotrophic yeast Pichia pastoris. // Cell Biol. Int. - 2011. – Vol.35, N4. – P.311-319.

2011

1. Abbas C. A., Sibirny A. A. Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers // Microbiol. Mol. Biol. Rev. – 2011. - Vol.75, № 2. - Р.321-360.
2. Grabek-Lejko D., Kurylenko O. O., Sibirny V. A., Ubiyvovk V. M., Penninckx M, Sibirny A. A. Alcoholic fermentation by wild-type Hansenula polymorpha and Saccharomyces cerevisiae versus recombinant strains with an elevated level of intracellular glutathione // J. Ind. Microbiol. Biotechnol. – 2011. – Vol.38, №11. - Р. 1853-1859.
3. Millerioux Y., Clastre M., Simkin A. J., Courdavault V., Marais E., Sibirny A. A., Noël T., Crèche J., Giglioli-Guivarcʼh N., Papon N. Drug-resistant cassettes for the efficient transformation of Candida guilliermondii wild-type strains // FEMS Yeast Res. – 2011. – Vol.11, №6. – Р.457-463.
4. Dmytruk K. V., Smutok O. V., Dmytruk O. V., Schuhmann W., Sibirny A. A. Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor // BMC Biotechnol. –2011. - Vol.11.- P.58-65.
5. Boretsky Y. R., Pynyaha Y. V., Boretsky V. Y., Fedorovych D. V., Fayura L. R, Protchenko O., Philpott C. C., Sibirny A. A. Identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast Pichia guilliermondii using insertion mutagenesis // FEMS Yeast Res. – 2011. - Vol.11, №3. Р. 307-314.
6. Ubiyvovk V. M., Ananin V. M., Malyshev A. Y., Kang H. A, Sibirny A. A. Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorpha DL-1 // BMC Biotechnol. – 2011. – Vol.11. P.8-19.
7. Millerioux Y., Clastre M., Simkin A. J., Marais E., Sibirny A. A., Noël T., Crèche J., Giglioli-Guivarcʼh N., Papon N. Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain // J. Microbiol. Methods. – 2011. - Vol.84, №2. Р.355-358.
8. Nazarko V. Y., Nazarko T. Y., Farré J. C., Stasyk O. V., Warnecke D., Ulaszewski S., Cregg J. M., Sibirny A. A., Subramani S. Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris // Autophagy. – 2011. Vol.7, №4. Р. 375-385.
9. Smutok O., Broda D., Smutok H., Dmytruk K., Gonchar M. Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b(2) // Chemosphere. – 2011. - Vol.83, №4. Р.449-454.
10. Sibirny A.. Metabolic engineering of non-conventional yeasts for construction of robust producers of fuels and chemicals // Nowoczene metody analizy surowcỏw rolniczych. Rzeszỏw: PAJ-Press. – 2011. - P. 445-453.
11. Sibirny W., Smutok O., Klepach H., Gayda G., Dmytruk K., Broda D., Gonchar M. Alcohol-selective amperometric biosensors based on natural and mutated alcohol oxidases // Nowoczene metody analizy surowcỏw rolniczych. Rzeszỏw: PAJ-Press. – 2011. - P. 241-253.
12. Grabek-Lejko D., Sibirny V., Kluz M., Sibirny A. Droźdźe niekonwencjonalne jako potencjialni producenci etanolu paliwowego z lignocelulozowych odpadỏw rolniczych. // Nowoczene metody analizy surowcỏw rolniczych. Rzeszỏw: PAJ-Press. – 2011. - P. 369-378.
13. Smutok O., Gayda G., Dmytruk K., Klepach H., Nisnevitch M., Sibirny A., Puchalski C., Broda D., Schuhmann W., Gonchar M., Sibirny V. Amperometric Biosensors for Lactate, Alcohols, and Glycerol Assays in Clinical Diagnostics // Biosensors - Emerging Materials and Applications. - 2011. – 630 р.
14. Sibirny A.A. Mechanisms of autophagy and pexophagy in yeasts // Biochemistry (Mosc). – 2011. – V. 76, No. 12. – P. 1279-1290.

2012

1. Sibirny A.A Molecular mechanisms of peroxisome biogenesis in yeasts // Molecular biology. – 2012. – Vol. 46, № 1. – P. 14-30.
2. Dmytruk K.V., Sibirny A.A. Candida famata (Candida flareri) // Yeast. – 2012. – V. 29, No. 11. – P. 453-458.
3. Klionsky D.J., Abdalla F.C., Abeliovich H., et al. Guidelines for the use and interpretation of assays for monitoring autophagy // Autophagy. – 2012. – V. 8, No. 4. – P. 445-544.
4. Polupanov A.S., Nazarko V.Y., Sibirny A.A.Gss1 protein of the methylotrophic yeast Pichia pastoris is involved in glucose sensing, pexophagy and catabolite repression // Int. J. Biochem. Cell. Biol. – 2012. – V. 44, No. 11. – P. 1906-1918.

2013

1. Prokopiv T.M., Fedorovych D.V., Boretsky Y.R., Sibirny A.A.Oversynthesis of riboflavin in the yeast Pichia guilliermondii is accompanied by reduced catalase and superoxide dismutases activities // Curr. Microbiol. – 2012. –V.66. – P.79-87.
2. Dmytruk K.V., Sibirny A.A. Metabolic engineering of yeast Hansenula polymorpha for construction of efficient ethanol producer // Cytology and Genetics (Tsitologiya i Genetika). – 2013. – Vol. 47, No. 6. – P. 3-21.
3. Fayura L.R., Boretsky Y.R., Pynyaha Y.V., Wheatley D.N., Sibirny A.A. Improved method for expression and isolation of the Mycoplasma hominis arginine deiminase from the recombinant strain of Escherichia coli // J. Biotechnol. – 2013. –Vol. 167, No. 4. P. 420-426.
4. Foureau E., Courdavault V., Simkin A.J., Sibirny A.A., Crèche J., Giglioli-Guivarcʼh N., Clastre M., Papon N. Transformation of Candida guilliermondii wild-type strains using the Staphylococcus aureus MRSA 252 ble gene as a phleomycin-resistant marker // FEMS Yeast Res. – 2013. – Vol. 13, No. 3. – P. 354-358.
5. Grabek-Lejko D., Sibirny V., Sibirny A. Regulation of gene expression in methylotrophic yeasts // Postepy Biochem. – 2013. – Vol. 59, No. 1. – P. 95-106. (Polish).
6. Krasovska O.S., Stasyk O.V., Sibirny A.A. Stable overproducer of hepatitis B surface antigen in the methylotrophic yeast Hansenula polymorpha due to multiple integration of heterologous auxotrophic selective markers and defect in peroxisome biogenesis // Appl. Microbiol. Biotechnol. – 2013. – Vol. 97, No. 23. – P. 9969-9979.
7. Naumova E.S., Dmitruk K.V., Kshanovskaya B.V., Sibirny A.A., Naumov G.I. Molecular identification of the industrially important strain Ogataea parapolymorpha // Microbiology (Mikrobiologiya). – 2013. – Vol. 82, No. 4. – P. 453-458.
8. Papon N., Savini V., Lanoue A., Simkin A.J., Crèche J., Giglioli-Guivarcʼh N., Clastre M., Courdavault V., Sibirny A.A. Candida guilliermondii: biotechnological applications, perspectives for biological control, emerging clinical importance and recent advances in genetics // Curr. Genet. – 2013. – Vol. 59, No. 3. – P. 73-90.
9. Polupanov A.S., Sibirny A.A. Cytoplasmic extension peptide of Pichia pastoris glucose sensor Gss1 is not compulsory for glucose signaling // Cell. Biol. Int. – 2013. – doi: 10.1002/cbin.10189.
10. Starokadomskyy P., Dmytruk K.V. A birdʼs-eye view of autophagy // Autophagy. – 2013. – Vol. 9, No. 7. - 121 – 1126.