Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Molecular Genetics and Biotechnology

The main research topics:

• Mechanisms of degradation of peroxisomal and cytosolic proteins in yeasts.


• Regulation of flavinogenesis in nonconventional yeasts. Cloning of structural and regulatory genes involved in biosynthesis of riboflavin and its derivatives (flavin coenzymes, roseoflavin) and its.


• Role of glutathione (GSH) in stress response of methylotrophic yeast cells: cloning of structural and regulatory genes, construction of GSH-overproducing strains, bioaccumulation of heavy metals.


• Production of biofuel ethanol by fermentation of lignocellulosic hydrolysates by non-conventional yeasts.


• Construction of microbial strains capable to produce isobutanol (yeasts) and hydrogen (bacteria).


• Biotechnological production of proteins important for medicine and diagnostics.


• Development of new combinatorial approaches for cancer treatment.

Institute of Cell Biology National Academy of Sciences of Ukraine

Department of Molecular Genetics and Biotechnology

Publications

2008

1. Dmytruk O. V., Dmytruk K. V., Abbas C. A., Voronovsky A. Y., Sibirny A. A. Engineering of xylose reductase and overexpression of xylitol dehydrogenase and xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha // Microb. Cell. Fact. - 2008. – №. 7. - P. 21.
2. Dmytruk O. V., Voronovsky A. Y., Abbas C. A., Dmytruk K. V., Ishchuk O. P., Sibirny A. A. Overexpression of bacterial xylose isomerase and own xylulokinase improves xylose alcoholic fermentation in the thermotolerant yeast Hansenula polymorpha // FEMS Yeast Res. – 2008. – Vol. 8, P. 165-173.
3. Ishchuk O. P., Dmytruk K. V., Rohulya O. V., Voronovsky A. Y., Abbas C. A., Sibirny A. A. Development Of A Promoter Assay System For The Flavinogenic Yeast Candida famata Based On The Kluyveromyces Lactis ß-Galactosidase Lac4 Reporter Gene. // Enzyme Microbial Technology. – 2008. – Vol. 42,N3. – P. 208-215.
4. Ishchuk O. P., Voronovsky A. Y., Stasyk O. V., Gayda G. Z., Gonchar M. V., Abbas C. A., Sibirny AA. Overexpression of pyruvate decarboxylase in the yeast Hansenula polymorpha results in increased ethanol yield in high-temperature fermentation of xylose. // FEMS Yeast Res. – 2008. – Vol. 8. – P. 1167-1174.
5. Ksheminska H., Fedorovych D., Honchar T., Ivash M., Gonchar M. Yeast tolerance to chromium depends on extra-cellular chromate reduction and Cr(III)-chelation // Food Technol. Biotechnol. - 2008. - Vol.46, №4. – P.420-427.
6. Lahtchev K. L., Batovska D. I., Parushev S. P., Ubiyvovk V. M., Sibirny A. A. Antifungal activity of chalcones: A mechanistic study using various yeast strains // Eur. J. Med. Chem. – 2008. - V.43, №10. - P. 2220-2228.
7. Nazarko V. Y., Thevelein J. M., Sibirny A. A. G-protein-coupled receptor Gpr1 and G-protein Gpa2 of cAMP-dependent signaling pathway are involved in glucose-induced pexophagy in the yeast Saccharomyces cerevisiae // Cell Biol. Int. – 2008. - № 32. – P. 502- 504.
8. Nazarko V. Y., Futej K. O., Thevelein J. M., Sibirny A. A. Differences in glucose sensing and signaling for pexophagy between the baker’s yeast Saccharomyces cerevisiae and the methylotrophic yeast Pichia pastoris // Autophagy. – 2008. - № 4. – P. 381- 384.
9. Dmitruk KV, Smutok OV, Gonchar MV, Sibirnyĭ AA. Construction of flavocytochrome b2-overproducing strains of the thermotolerant methylotrophic yeast Hansenula polymorpha (Pichia angusta) // Mikrobiologia (Moscow). – 2008. - V.77. – P.213-218. (in Russian).
10. Dmytruk O.V., Dmytruk K.V., Voronovsky A.Y., Sibirny A.A. Metabolic engineering of the initial stages of xylose catabolism in yeasts for construction of efficient producers of ethanol from lignocelluloses // Tsitologiia i genetika (Cytology and Genetics) – 2008. – Vol..42. – P.127-138.
11. Sibirny V.А., Gonchar М.V., Grabek-Lejko D., Pavlishko H.М., Csöregi E., Sibirny A.A. Photometric assay of methanol and formaldehyde in industrial waste-waters using alcohol oxidase and 3-methyl-2-benzothiazolinone hydrazone // Intern. J. Environ. Anal. Chem. – 2008. – V. 88, N 4. – P. 289-301.
12. Sibirny A. A., Voronovsky A. Y. Chapter 5: Candida famata (Debaryomyces hansenii). In: Yeast biotechnology: diversity and applications (G. Kunze, T. Satyanarayana Eds), Springer Science. 2008; Р. 83-110.
13. Sibirny А.А, Boretsky Y.R. Chapter 6: Pichia guilliermondii. In: Yeast biotechnology: diversity and applications (G. Kunze, T. Satyanarayana Eds), Springer Science. 2008; РР 111-132.
14. Klionsky D.J. et al. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes (Review)// Autophagy. – V.4, №2. – P.151-175.
15. Stasyk O.G., Maidan M. M., Stasyk O. V., Van Dijck P., Thevelein J.M., Sibirny A.A. Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha. Eukaryotic cell. – 2008. – Vol.7(4). – P.735-746.

2009

1. Yatsyshyn, V.Y., Fedorovych, D.V., Sibirny, A.A. The microbial synthesis of flavin nucleotides: a review // Appl. Biochem. Microbiol. – 2009. – Vol. 45, No 2. – Р. 115–124.
2. Pynyaha YV, Boretsky YR, Fedorovych DV, Fayura LR, Levkiv AI, Ubiyvovk VM, Protchenko OV, Philpott CC, Sibirny AA. Deficiency in frataxin homologue YFH1 in the yeast Pichia guilliermondii leads to missregulation of iron acquisition and riboflavin biosynthesis and affects sulfate assimilation // Biometals. – 2009. – Vol.22. - P.1051-1061.
3. Cheon SA, Choo J, Ubiyvovk VM, Park JN, Kim MW, Oh DB, Kwon O, Sibirny AA, Kim JY, Kang HA. New selectable host-marker systems for multiple genetic manipulations based on TRP1, MET2 and ADE2 in the methylotrophic yeast Hansenula polymorpha // Yeast. – 2009. – Vol. 26, N9. - P.507- 521.
4. Voronovsky AY, Rohulya OV, Abbas CA, Sibirny AA. Development of strains of the thermotolerant yeast Hansenula polymorpha capable of alcoholic fermentation of starch and xylan. // Metabolic Engineering. – 2009. – Vol. 11, N4-5. – P. 234-242.
5. Ishchuk OP, Voronovsky AY, Abbas CA, Sibirny AA. Construction of Hansenula polymorpha strains with improved thermotolerance // Biotechnol. Bioeng. – 2009. – Vol.104, N5. – P. 911-919.
6. Yatsyshyn VY, Ishchuk OP, Voronovsky AY, Fedorovych DV, Sibirny AA. Production of flavin mononucleotide by metabolically engineered yeast Candida famata. // Metabolic Engineering. – 2009. Vol.11, N3. – P. 163-167.
7. Shkil H, Stoica L, Dmytruk K, Smutok O, Gonchar M, Sibirny A, Schuhmann W. Bioelectrochemical detection of L-lactate respiration using genetically modified Hansenula polymorpha yeast cells overexpressing flavocytochrome b(2) // Bioelectrochemistry. 2009, 76(1-2):175-9
8. Fedorovych D., Gonchar M., Ksheminska H., Prokopiv T., Nechay H., Kaszycki P., Koloczek H., Sibirny A. Mechanisms of chromate detoxification in yeasts // Miсrobiology and Biotechnology. - 2009. – №.7. – С.15-21

2010

1. Bobak Y.P., Vynnytska B.O., Kurlishchuk Y.V., Sibirny A.A., Stasyk O.V. Cancer cell sensitivity to arginine deprivation in vitro is not determined by endogenous levels of arginine metabolic enzymes // Cell Biol Int. – 2010. - Vol.34, №11. - P.1085-1089.
2. Ishchuk O.P., Abbas C.A., Sibirny A.A. Heterologous expression of Saccharomyces cerevisiae MPR1 gene confers tolerance to ethanol and L: -azetidine-2-carboxylic acid in Hansenula polymorpha. J Ind. Microbiol .Biotechnol. - 2010. – Vol.37, N2. P. 213-218.
3. Dmytruk K.V., Yatsyshyn V.Y., Sibirna N.O., Fedorovych D.V., Sibirny A.A. Metabolic engineering and classic selection of the yeast Candida famata (Candida flareri) for construction of strains with enhanced riboflavin production // Metabolic Engineering. – 2011. - Vol.13. – P.33-38.
4. Yatsyshyn V.Y., Fedorovych D.V., Sibirny A.А. Medium optimization for production of flavin mononucleotide by the recombinant strain of the yeast Candida famata strain using statistical designs // Biochemical Engineering Journal. - 2010. - Vol.49, P.52-60.
5. Sybirna K, Petryk N, Zhou YF, Sibirny A, Bolotin-Fukuhara M. A novel Hansenula polymorpha transcriptional factor HpHAP4-B, able to functionally replace the S. cerevisiae HAP4 gene, contains an additional bZip motif. // Yeast. - 2010. – Vol. 27, №11. - P.941-954.
6. Polupanov A.S., Nazarko V.Y., Sibirny A.A. CCZ1, MON1 and YPT7 genes are involved in pexophagy, Cvt pathway and non-specific macroautophagy in the methylotrophic yeast Pichia pastoris. // Cell Biol. Int. - 2011. – Vol.35, N4. – P.311-319.

2011

1. Abbas C. A., Sibirny A. A. Genetic control of biosynthesis and transport of riboflavin and flavin nucleotides and construction of robust biotechnological producers // Microbiol. Mol. Biol. Rev. – 2011. - Vol.75, № 2. - Р.321-360.
2. Grabek-Lejko D., Kurylenko O. O., Sibirny V. A., Ubiyvovk V. M., Penninckx M, Sibirny A. A. Alcoholic fermentation by wild-type Hansenula polymorpha and Saccharomyces cerevisiae versus recombinant strains with an elevated level of intracellular glutathione // J. Ind. Microbiol. Biotechnol. – 2011. – Vol.38, №11. - Р. 1853-1859.
3. Millerioux Y., Clastre M., Simkin A. J., Courdavault V., Marais E., Sibirny A. A., Noël T., Crèche J., Giglioli-Guivarcʼh N., Papon N. Drug-resistant cassettes for the efficient transformation of Candida guilliermondii wild-type strains // FEMS Yeast Res. – 2011. – Vol.11, №6. – Р.457-463.
4. Dmytruk K. V., Smutok O. V., Dmytruk O. V., Schuhmann W., Sibirny A. A. Construction of uricase-overproducing strains of Hansenula polymorpha and its application as biological recognition element in microbial urate biosensor // BMC Biotechnol. –2011. - Vol.11.- P.58-65.
5. Boretsky Y. R., Pynyaha Y. V., Boretsky V. Y., Fedorovych D. V., Fayura L. R, Protchenko O., Philpott C. C., Sibirny A. A. Identification of the genes affecting the regulation of riboflavin synthesis in the flavinogenic yeast Pichia guilliermondii using insertion mutagenesis // FEMS Yeast Res. – 2011. - Vol.11, №3. Р. 307-314.
6. Ubiyvovk V. M., Ananin V. M., Malyshev A. Y., Kang H. A, Sibirny A. A. Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorpha DL-1 // BMC Biotechnol. – 2011. – Vol.11. P.8-19.
7. Millerioux Y., Clastre M., Simkin A. J., Marais E., Sibirny A. A., Noël T., Crèche J., Giglioli-Guivarcʼh N., Papon N. Development of a URA5 integrative cassette for gene disruption in the Candida guilliermondii ATCC 6260 strain // J. Microbiol. Methods. – 2011. - Vol.84, №2. Р.355-358.
8. Nazarko V. Y., Nazarko T. Y., Farré J. C., Stasyk O. V., Warnecke D., Ulaszewski S., Cregg J. M., Sibirny A. A., Subramani S. Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris // Autophagy. – 2011. Vol.7, №4. Р. 375-385.
9. Smutok O., Broda D., Smutok H., Dmytruk K., Gonchar M. Chromate-reducing activity of Hansenula polymorpha recombinant cells over-producing flavocytochrome b(2) // Chemosphere. – 2011. - Vol.83, №4. Р.449-454.
10. Sibirny A.. Metabolic engineering of non-conventional yeasts for construction of robust producers of fuels and chemicals // Nowoczene metody analizy surowcỏw rolniczych. Rzeszỏw: PAJ-Press. – 2011. - P. 445-453.
11. Sibirny W., Smutok O., Klepach H., Gayda G., Dmytruk K., Broda D., Gonchar M. Alcohol-selective amperometric biosensors based on natural and mutated alcohol oxidases // Nowoczene metody analizy surowcỏw rolniczych. Rzeszỏw: PAJ-Press. – 2011. - P. 241-253.
12. Grabek-Lejko D., Sibirny V., Kluz M., Sibirny A. Droźdźe niekonwencjonalne jako potencjialni producenci etanolu paliwowego z lignocelulozowych odpadỏw rolniczych. // Nowoczene metody analizy surowcỏw rolniczych. Rzeszỏw: PAJ-Press. – 2011. - P. 369-378.
13. Smutok O., Gayda G., Dmytruk K., Klepach H., Nisnevitch M., Sibirny A., Puchalski C., Broda D., Schuhmann W., Gonchar M., Sibirny V. Amperometric Biosensors for Lactate, Alcohols, and Glycerol Assays in Clinical Diagnostics // Biosensors - Emerging Materials and Applications. - 2011. – 630 р.
14. Sibirny A.A. Mechanisms of autophagy and pexophagy in yeasts // Biochemistry (Mosc). – 2011. – V. 76, No. 12. – P. 1279-1290.

2012

1. Sibirny A.A Molecular mechanisms of peroxisome biogenesis in yeasts // Molecular biology. – 2012. – Vol. 46, № 1. – P. 14-30.
2. Dmytruk K.V., Sibirny A.A. Candida famata (Candida flareri) // Yeast. – 2012. – V. 29, No. 11. – P. 453-458.
3. Klionsky D.J., Abdalla F.C., Abeliovich H., et al. Guidelines for the use and interpretation of assays for monitoring autophagy // Autophagy. – 2012. – V. 8, No. 4. – P. 445-544.
4. Polupanov A.S., Nazarko V.Y., Sibirny A.A.Gss1 protein of the methylotrophic yeast Pichia pastoris is involved in glucose sensing, pexophagy and catabolite repression // Int. J. Biochem. Cell. Biol. – 2012. – V. 44, No. 11. – P. 1906-1918.

2013

1. Prokopiv T.M., Fedorovych D.V., Boretsky Y.R., Sibirny A.A.Oversynthesis of riboflavin in the yeast Pichia guilliermondii is accompanied by reduced catalase and superoxide dismutases activities // Curr. Microbiol. – 2012. –V.66. – P.79-87.
2. Dmytruk K.V., Sibirny A.A. Metabolic engineering of yeast Hansenula polymorpha for construction of efficient ethanol producer // Cytology and Genetics (Tsitologiya i Genetika). – 2013. – Vol. 47, No. 6. – P. 3-21.
3. Fayura L.R., Boretsky Y.R., Pynyaha Y.V., Wheatley D.N., Sibirny A.A. Improved method for expression and isolation of the Mycoplasma hominis arginine deiminase from the recombinant strain of Escherichia coli // J. Biotechnol. – 2013. –Vol. 167, No. 4. P. 420-426.
4. Foureau E., Courdavault V., Simkin A.J., Sibirny A.A., Crèche J., Giglioli-Guivarcʼh N., Clastre M., Papon N. Transformation of Candida guilliermondii wild-type strains using the Staphylococcus aureus MRSA 252 ble gene as a phleomycin-resistant marker // FEMS Yeast Res. – 2013. – Vol. 13, No. 3. – P. 354-358.
5. Grabek-Lejko D., Sibirny V., Sibirny A. Regulation of gene expression in methylotrophic yeasts // Postepy Biochem. – 2013. – Vol. 59, No. 1. – P. 95-106. (Polish).
6. Krasovska O.S., Stasyk O.V., Sibirny A.A. Stable overproducer of hepatitis B surface antigen in the methylotrophic yeast Hansenula polymorpha due to multiple integration of heterologous auxotrophic selective markers and defect in peroxisome biogenesis // Appl. Microbiol. Biotechnol. – 2013. – Vol. 97, No. 23. – P. 9969-9979.
7. Naumova E.S., Dmitruk K.V., Kshanovskaya B.V., Sibirny A.A., Naumov G.I. Molecular identification of the industrially important strain Ogataea parapolymorpha // Microbiology (Mikrobiologiya). – 2013. – Vol. 82, No. 4. – P. 453-458.
8. Papon N., Savini V., Lanoue A., Simkin A.J., Crèche J., Giglioli-Guivarcʼh N., Clastre M., Courdavault V., Sibirny A.A. Candida guilliermondii: biotechnological applications, perspectives for biological control, emerging clinical importance and recent advances in genetics // Curr. Genet. – 2013. – Vol. 59, No. 3. – P. 73-90.
9. Polupanov A.S., Sibirny A.A. Cytoplasmic extension peptide of Pichia pastoris glucose sensor Gss1 is not compulsory for glucose signaling // Cell. Biol. Int. – 2013. – doi: 10.1002/cbin.10189.
10. Starokadomskyy P., Dmytruk K.V. A birdʼs-eye view of autophagy // Autophagy. – 2013. – Vol. 9, No. 7. - 121 – 1126.

2014

1. Dmytruk K., Lyzak O., Yatsyshyn V., Kluz M., Sibirny V., Puchalski C., Sibirny A. Construction and fed-batch cultivation of Candida famata with enhanced riboflavin production // Journal of Biotechnology. – 2014. – Vol. 172. – P. 11-17. (IF=2.900)
2. Kurylenko O., Ruchala J., Hryniv O., Abbas C., Dmytruk K., Sibirny A. Metabolic engineering and classic selection of the methylotrophic thermotolerant yeast Hansenula polymorpha for improvement of high-temperature xylose alcoholic fermentation // Microbial Cell Factories – 2014. – Vol.13, No.122 (IF=4.300)
3. Rachkevych N., Sybirna K., Boyko S., Boretsky Y., Sibirny A. Improving the efficiency of plasmid transformation in Shewanella oneidensis MR-1 by removing ClaI restriction site // J Microbiol Methods. – 2014. – Vol. 99. – P.35-37. (IF=2.300)
4. Semkiv M.V., Dmytruk K.V., Abbas C.A., Sibirny A.A. Increased ethanol accumulation from glucose via reduction of ATP level in a recombinant strain of Saccharomyces cerevisiae overexpressing alkaline phosphatase // BMC Biotechnol. – 2014. – Vol. 14, No. 1. – P. 42-47. (IF=2.600)
5. Polupanov A.S., Sibirny A.A. Cytoplasmic extension peptide of Pichia pastoris glucose sensor Gss1 is not compulsory for glucose signaling // Cell. Biol. Int. – 2013. – Volume 38, Issue 2, February 2014, Pages: 172–178 (IF=1.640)
6. Yatsyshyn V.Y., Fedorovych D.V., Sibirny A.A. Metabolic and bioprocess engineering of the yeast Candida famata for FAD production // Journal of industrial microbiology & biotechnology. – 2014. – Vol. 41, No. 5. – P. 823-835. (IF=2.500)
7. *Smutok O.V., Dmytruk K.V., Karkovska M.I., Schuhmann W., Gonchar M.V., Sibirny A.A. D-lactate-selective amperometric biosensor based on the cell debris of the recombinant yeast Hansenula polymorpha // Talanta. – 2014. – V. 125. - P. 227-232 (IF=3.511)
8. Фаюра Л.Р., Борецький Ю.Р., Пиняга Ю.В., Мартинюк Н.Б., Скороход В.В., Сибірний А.А.. Розробка технології культивування рекомбінантного штаму-продуцента Escherichia coli з метою отримання аргініндезімінази Mycoplasma hominis // Наука та інновації. – 2014. – Т.10, №4. – С.32-39.
9. Дмитрук О.В., Сибірний А.А. Катаболітна деградація фруктозо-1,6-бісфосфатази у метилотрофних дріжджів Pichia pastoris і Hansenula polymorpha // Фактори експериментальної еволюції організмів: Зб. наук. пр. / Укр. т-во генетиків і селекціонерів ім. М.І. Вавилова. – К., 2014. – Т.15, С. 55–59.
10. Лужецький Т.Б., Семків М.В., Кшановська Б.В., Дмитрук К.В., Сибірний А.А. Підвищення термотолерантності промислових спиртових дріжджів шляхом дерепресії генів синтезу трегалози // Фактори експериментальної еволюції організмів: Зб. наук. пр. / Укр. т-во генетиків і селекціонерів ім. М.І. Вавилова. – К., 2014. – Т.15, С. 95-99.
11. *Sibirny A.A. Pexophagy Sensing and Signaling in the Methylotrophic Yeasts // Molecular Machines Involved in Peroxisome Biogenesis and Maintenance / Ed.by C. Brocard and A. Hartig. - Springer-Verlag Wien. - 2014. – P. 507-527, 1,2 ум.друк арк.
12. *Sibirny A., Madzak C., Fickers P. Genetic engineering of nonconventional yeasts for the production of valuable compounds // Microbial Biotechnology: Progress and Trends / Ed. by F.D. Harzevili and H. Chen. – Springer. – 2014. - Chapter 4. – P. 62-109, 2,22 ум.друк.арк.

2015

1. Dmytruk K., Kshanovska B., Abbas C., Sibirny A. New methods for positive selection of yeast ethanol overproducing mutants // Bioethanol.- 2015.- Vol. 2. P. 24–31.
2. Fedorovych D., Boretsky Y., Bobak Ya., Prokopiv T., Sybirny A. Putative ferroxidases in the flavinogenic yeast Pichia guilliermondii are regulated by iron acquisition. // Tsitologiia i genetika (Cytology and Genetics). – 2015. – Vol.49, No.5 – P. 13-19. (IF=0.400)
3. *Stasyk O.V., Boretsky Y.R., Gonchar M.V. and Sibirny A.A. Recombinant arginine-degrading enzymes in metabolic anticancer therapy and bioanalytics. // Cell Biology International. – 2015 – Vol. 39, No.3 – P. 246–252. (IF=1.933)
4. Tangsombatvichit P., Semkiv M.V., Sibirny A.A., Jensen L.T., Ratanakhanokchai K., Soontorngun N. Zinc Cluster Protein Znf1, a Novel Transcription Factor of Non-Fermentative Metabolism in Saccharomyces cerevisiae. // FEMS Yeast Res. – 2015. – Vol. 15, No. 2. – doi: 10.1093/femsyr/fou002. (IF=2.818)
5. Юрків М.Т., Куриленко О.О., Василишин Р.В., Дмитрук К.В., Мартинюк Н.Б., Скороход В.В., Сибірний А.А. Pозробка технології отримання препаратів глутатіону на основі сконструйованих активних суперпродуцентів цього трипептиду у дріжджів. // Nauka innov. – 2015 – 11 (5): C. 67—75.
6. Semkiv M., Kurylenko O., Ruchala J., Hryniv O., Kshanovska B., Dmytruk K., Sibirny A. Yeast Alcoholic Fermentation: Achievements and Challenges. // Modern Directions in Chemistry, Biology, Pharmacy and Biotechnology: Monograph. / Lviv Polytechnic Publishing House. – Lviv, 2015. – P. 235-248.
7. *Berezka K., Sibirny A. Mercury: From General Informations, Through Microbial Interactions to Bioremediation. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 11-20.
8. *Dmytruk K., Kshanovska B., Sibirny A. New Methods for Selection of Saccharomyces cerevisiae Ethanol Overproducing Mutants Resistant to Oxythiamine, Trehalose, Bromopyruvate, Glyoxylic Acid and Glucosamine. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 237-246.
9. *Fedorovych D., Boretsky Y., Prokopiv T., Grabek-Lejko D., Sybirny A. Cobalt as a Dangerous Environmental Pollutant. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 33-40.
10. *Karkovska M., Smutok O., Dmytruk K., Gonchar M. Yeast Flavocytochrome b2 as a Perspective Tool in Bioreduction and Detection of Chromate. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 113-124.
11. *Kluz M., Sibirny A.A. Сhromium − Toxicity and Bioremediation by Microorganisms. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 41-50.
12. *Kurylenko O., Ruchala J., Dmytruk K., Sibirny A. New Targets for Improvement of Xylose Alcoholic Fermentation in the Methylotrophic Yeast Hansenula polymorpha. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 247-258.
13. *Luzhetskyi T., Semkiv M., Dmytruk K., Sibirny A. Improving Thermotolerance of Saccharomyces cerevisiae Industrial Yeast Strain via Derepression of Genes of Trehalose Synthesis. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 259-268.
14. *Lyzak O., Semkiv M., Dmytruk K., Sibirny A. Targets of Transcription Factor Sef1 in the Flavinogenic Yeasts Candida famata. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 269-278.
15. *Rachkevych N., Boyko S., Sybirna K., Boretsky Y., Sibirny A. The Development of Shewanella oneidensis MR-1 as a Host for Hydrogenase Overexpression. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 279-290.
16. *Ruchala J., Kurylenko O., Sibirny A. Production of Glutathione by Yeasts and the Role of this Thiol in Methylotrophic Metabolism and Xenobiotic Detoxification. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 227-236.
17. *Salii I., Kurylenko O., Hryniv O., Dmytruk K., Sibirny A. Microbial Conversion of Glycerol to Ethanol. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 291-300.
18. *Semkiv M., Dmytruk K., Sibirny A. Futile Cycles Induction as a Tool for Improvement of Alcoholic Fermentation Parameters in Saccharomyces cerevisiae. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 301-312.
19. *Semkiv M., Dmytruk K., Sibirny A. Impact of Triose Phosphate Isomerase Activity Modulation on Glycerol Production by the Yeast Saccharomyces cerevisiae. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 313-322.
20. *Semkiv M., Kurylenko O., Ruchala J., Hryniv O., Dmytruk K., Sibirny A. Yeast Metabolic Engineering for Construction of the Advanced Bioethanol Producers from Renewable Feedstocks. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 333-341.
21. *Yatsyshyn V., Fedorovych D., Sibirny A. The Role of Flavin Nucleotides in Detoxification of Pollutants in the Natural Environment. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 187-194.
22. *Yurkiv M., Kurylenko O., Vasylyshyn R., Dmytruk K., Sibirny A. Construction of the Efficient Glutathione Producers in the Yeast Hansenula polymorpha. // Living Organisms and Bioanalytical Approaches for Detoxification and Monitoring of Toxic Compounds: Monograph. / University of Rzeszow. – Rzeszow, 2015. – P. 323-332.
23. *Dmytruk K., Sibirny A. Insertional Mutagenesis of the Flavinogenic Yeast Candida famata (Candida flareri) / in Genetic transformation system in fungi, Vol. 1, Fungal biology. – 2015. – Part III. – P. 93-97.
24. *Papon N., Boretsky Y.R., Courdavault V., Clastre M., Sibirny A.A. Genetic manipulation of Meyerozyma (Candida, Pichia) guilliermondii / in Genetic transformation system in fungi, Vol. 2, Fungal biology. – 2015. – Part VI. – P. 245-262.
25. Semkiv M., Kurylenko O., Ruchala J., Hryniv O., Kshanovska B., Dmytruk K., Sibirny A. Yeast Alcoholic Fermentation: Achievements and Challenges. // Modern Directions in Chemistry, Biology, Pharmacy and Biotechnology: Monograph. / Lviv Polytechnic Publishing House. – Lviv, 2015. – P. 235-248.